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Literature summary for 2.5.1.66 extracted from

  • Caines, M.E.; Sorensen, J.L.; Schofield, C.J.
    Structural and mechanistic studies on N(2)-(2-carboxyethyl)arginine synthase (2009), Biochem. Biophys. Res. Commun., 385, 512-517.
    View publication on PubMed
Search for more literature for 2.5.1.66

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structures of tetrameric CEAS-thiamin diphosphate in complex with the substrate analogues 5-guanidinovaleric acid and tartrate, crystals of 2 morphologies are grown by the hanging-drop vapour diffusion technique using 1.6 M ammonium sulfate as precipitant, in the presence of 0.1 M HEPES, pH 7.4, and a 3fold molar excess of thiamin diphosphate Streptomyces clavuligerus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde 3-phosphate + L-arginine Streptomyces clavuligerus
-
 N2-(2-carboxyethyl)-L-arginine + phosphate N2-(2-carboxyethyl)-L-arginine is the immediate precursor to the first beta-lactam during the biosynthesis of the clinically important serine beta-lactamase inhibitor, clavulanic acid, in Streptomyces clavuligerus ?
D-glyceraldehyde 3-phosphate + L-lysine Streptomyces clavuligerus
-
 N2-(2-carboxyethyl)-L-lysine + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces clavuligerus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + L-arginine
-
 Streptomyces clavuligerus N2-(2-carboxyethyl)-L-arginine + phosphate N2-(2-carboxyethyl)-L-arginine is the immediate precursor to the first beta-lactam during the biosynthesis of the clinically important serine beta-lactamase inhibitor, clavulanic acid, in Streptomyces clavuligerus ?
D-glyceraldehyde 3-phosphate + L-lysine
-
 Streptomyces clavuligerus N2-(2-carboxyethyl)-L-lysine + phosphate
-
 ?

Subunits

Subunits Comment Organism
tetramer determined by cristallization experiments Streptomyces clavuligerus

Synonyms

Synonyms Comment Organism
CEAS
-
 Streptomyces clavuligerus
N(2)-(2-carboxyethyl)arginine synthase
-
 Streptomyces clavuligerus

General Information

General Information Comment Organism
physiological function enzyme catalyses the committed step in the biosynthesis of the beta-lactamase inhibitor clavulanic acid Streptomyces clavuligerus