Literature summary for 2.5.1.7 extracted from

Skarzynski, T.; Mistry, A.; Wonacott, A.; Hutchinson, S.E.; Kelly, V.A.; Duncan, K.
Structure of UDP-N-acetylglucosamine enolpyruvyl transferase, and enzyme essential for the synthesis of bacterial peptidoglycan, complexed with substrate UDP-N-acetylglucosamine and the drug fosfomycin (1996), Structure, 4, 1465-1474.

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Application

Application	Comment	Organism
medicine	enzyme is a target for the antibiotic fosfomycin	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystallization of the enzyme complexed with UDP-N-acetylglucosamine and fosfomycin, two-domain structure with the active site located between them, structure and substrate binding analysis	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
fosfomycin	binds covalently to Cys115	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine	Escherichia coli	enzyme catalyzes the first committed step in the biosynthesis of bacterial cell wall peptidoglycan	phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	gene murA	-

Reaction

Reaction	Comment	Organism	Reaction ID
phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine	Cys115 is the active site nucleophile	Escherichia coli	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine	substrate binding structure	Escherichia coli	phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine	-	?
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine	enzyme catalyzes the first committed step in the biosynthesis of bacterial cell wall peptidoglycan	Escherichia coli	phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine	-	?

Subunits

Subunits	Comment	Organism
More	two-domain structure with the active site located between them, substrate binding structure	Escherichia coli

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Release 2023.1 (January 2023)