Any feedback?
Literature summary for 2.5.1.7 extracted from
- Determination of the pKa value of C115 in MurA (UDP-N-acetylglucosamine enolpyruvyltransferase) from Enterobacter cloacae (2000), Biochemistry, 39, 12671-12677.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C115S | - |
Enterobacter cloacae |
| C251S | site-directed mutagenesis, Cys251 is not involved in the catalysis, unaltered biochemical properties | Enterobacter cloacae |
| C354S | site-directed mutagenesis, Cys354 is not involved in the catalysis, unaltered biochemical properties | Enterobacter cloacae |
| C381S | site-directed mutagenesis, Cys381 is not involved in the catalysis, unaltered biochemical properties | Enterobacter cloacae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| fosfomycin | binds covalently to Cys115 | Enterobacter cloacae |  |
| iodoacetamide | inhibition by alkylation of the active site Cys155, pH-dependent, no alkylation below pH 7.0, maximum alkylation at pH 9.0 | Enterobacter cloacae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km values of mutant enzymes for the substrates | Enterobacter cloacae | |
| 0.008 | - |
phosphoenolpyruvate | wild-type enzyme | Enterobacter cloacae | |
| 0.08 | - |
UDP-GlcNAc | wild-type enzyme | Enterobacter cloacae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterobacter cloacae | - |
MurA | - |
| Enterobacter cloacae | - |
recombinant purified enzyme | - |
| Enterobacter cloacae DSM 30054 | - |
MurA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| covalent adduct of enzyme and phosphoenolpyruvate | Enterobacter cloacae |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine | mechanism | Enterobacter cloacae | |
| phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine | Cys115 in the active site can act as proton donor, necessary for activity, or as a nucleophile | Enterobacter cloacae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | - |
Enterobacter cloacae | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | - |
r | |
| phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | - |
Enterobacter cloacae DSM 30054 | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UDP-N-acetylglucosamine enolpyruvyltransferase | - |
Enterobacter cloacae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kcat values of mutant enzymes for the substrates | Enterobacter cloacae | |
| 3 | - |
phosphoenolpyruvate | wild-type enzyme | Enterobacter cloacae | |
| 3 | - |
UDP-GlcNAc | wild-type enzyme | Enterobacter cloacae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
determination of pKa value for Cys115: 8.3 | Enterobacter cloacae |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
