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Literature summary for 2.5.1.7 extracted from
- Characterization of mycobacterial UDP-N-acetylglucosamine enolpyruvyle transferase (MurA) (2014), Res. Microbiol., 165, 91-101.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 cells | Mycolicibacterium smegmatis |
| expressed in Escherichia coli BL21 cells | Mycobacterium tuberculosis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.121 | - |
phosphoenolpyruvate | at pH 8.0 and 37°C | Mycobacterium tuberculosis |  |
| 0.199 | - |
phosphoenolpyruvate | at pH 7.5 and 37°C | Mycolicibacterium smegmatis | |
| 2.32 | - |
UDP-N-acetyl-alpha-D-glucosamine | at pH 8.0 and 37°C | Mycobacterium tuberculosis | |
| 2.743 | - |
UDP-N-acetyl-alpha-D-glucosamine | at pH 7.5 and 37°C | Mycolicibacterium smegmatis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is independent of Mg2+ and Ca2+ | Mycolicibacterium smegmatis | |
| additional information | the enzyme is independent of Mg2+ and Ca2+ | Mycobacterium tuberculosis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45430 | - |
x * 45500, calculated from amino acid sequence | Mycolicibacterium smegmatis |
| 45500 | - |
- |
Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine | Mycolicibacterium smegmatis | - |
phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine | - |
? | |
| phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine | Mycobacterium tuberculosis | - |
phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine | - |
? | |
| phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine | Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | - |
phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine | - |
? | |
| phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine | Mycobacterium tuberculosis H37Rv | - |
phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
| Mycobacterium tuberculosis H37Rv | - |
- |
- |
| Mycolicibacterium smegmatis | - |
- |
- |
| Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Mycolicibacterium smegmatis |
| Ni-NTA column chromatography | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine | - |
Mycolicibacterium smegmatis | phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine | - |
? | |
| phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine | - |
Mycobacterium tuberculosis | phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine | - |
? | |
| phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine | - |
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine | - |
? | |
| phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine | - |
Mycobacterium tuberculosis H37Rv | phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45500, calculated from amino acid sequence | Mycolicibacterium smegmatis |
| ? | x * 45430, calculated from amino acid sequence | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MurA | - |
Mycolicibacterium smegmatis |
| MurA | - |
Mycobacterium tuberculosis |
| UDP-N-acetylglucosamine enolpyruvyle transferase | - |
Mycolicibacterium smegmatis |
| UDP-N-acetylglucosamine enolpyruvyle transferase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Mycolicibacterium smegmatis |
| 37 | - |
- |
Mycobacterium tuberculosis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.033 | - |
UDP-N-acetyl-alpha-D-glucosamine | at pH 7.5 and 37°C | Mycolicibacterium smegmatis | |
| 0.058 | - |
phosphoenolpyruvate | at pH 7.5 and 37°C | Mycolicibacterium smegmatis | |
| 0.117 | - |
phosphoenolpyruvate | at pH 8.0 and 37°C | Mycobacterium tuberculosis | |
| 0.147 | - |
UDP-N-acetyl-alpha-D-glucosamine | at pH 8.0 and 37°C | Mycobacterium tuberculosis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Mycolicibacterium smegmatis |
| 8 | - |
- |
Mycobacterium tuberculosis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | murA is an essential gene for growth of Mycobacterium smegmatis | Mycolicibacterium smegmatis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0119 | - |
UDP-N-acetyl-alpha-D-glucosamine | at pH 7.5 and 37°C | Mycolicibacterium smegmatis | |
| 0.0636 | - |
UDP-N-acetyl-alpha-D-glucosamine | at pH 8.0 and 37°C | Mycobacterium tuberculosis | |
| 0.2929 | - |
phosphoenolpyruvate | at pH 7.5 and 37°C | Mycolicibacterium smegmatis | |
| 0.9636 | - |
phosphoenolpyruvate | at pH 8.0 and 37°C | Mycobacterium tuberculosis | |
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