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Literature summary for 2.5.1.7 extracted from

  • Sonkar, A.; Shukla, H.; Shukla, R.; Kalita, J.; Pandey, T.; Tripathi, T.
    UDP-N-Acetylglucosamine enolpyruvyl transferase (MurA) of Acinetobacter baumannii (AbMurA) Structural and functional properties (2017), Int. J. Biol. Macromol., 97, 106-114 .
    View publication on PubMed
Search for more literature for 2.5.1.7

Cloned(Commentary)

Cloned (Comment) Organism
for the expression of AbMurA, the plasmid is introduced into C41(DE3) cells Acinetobacter baumannii

Inhibitors

Inhibitors Comment Organism Structure
fosfomycin 50 mM, 3fold inhibition Acinetobacter baumannii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.062
-
 UDP-N-acetyl-alpha-D-glucosamine pH 7.5, 37°C Acinetobacter baumannii
1.806
-
 phosphoenolpyruvate pH 7.5, 37°C Acinetobacter baumannii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 gel filtration Acinetobacter baumannii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine Acinetobacter baumannii the enzyme catalyzes the first committed step of peptidoglycan biosynthesis phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine
-
 ?

Organism

Organism UniProt Comment Textmining
Acinetobacter baumannii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Acinetobacter baumannii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine
-
 Acinetobacter baumannii phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine
-
 ?
phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine the enzyme catalyzes the first committed step of peptidoglycan biosynthesis Acinetobacter baumannii phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 45000, SDS-PAGE Acinetobacter baumannii

Synonyms

Synonyms Comment Organism
MurA
-
 Acinetobacter baumannii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Acinetobacter baumannii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Acinetobacter baumannii

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
11.7
-
 pH 7.5, 37°C Acinetobacter baumannii fosfomycin

General Information

General Information Comment Organism
drug target the lack of a counterparts in eukaryotes suggests the enzyme to be a promising drug target Acinetobacter baumannii
metabolism the enzyme catalyzes the first committed step of peptidoglycan biosynthesis. MurA is indispensable for cell integrity Acinetobacter baumannii