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Literature summary for 2.5.1.73 extracted from

  • Hauenstein, S.I.; Perona, J.J.
    Redundant synthesis of cysteinyl-tRNACys in Methanosarcina mazei (2008), J. Biol. Chem., 283, 22007-22017.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta2(DE3) pLysS cells Methanosarcina mazei
expression in Escherichia coli Methanosarcina mazei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
2 * 42000, gel filtration Methanosarcina mazei
42324
-
2 * 42324, predicted from amino acid sequence Methanosarcina mazei
84000
-
gel filtration Methanosarcina mazei

Organism

Organism UniProt Comment Textmining
Methanosarcina mazei
-
-
-
Methanosarcina mazei Q8PVS9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanosarcina mazei
DEAE column chromatography and Superdex 200 gel filtration Methanosarcina mazei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
highly sensitive assay to measure the activity of SepCysS under anaerobic conditions Methanosarcina mazei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information thiosulfate shows 72.6% of the activity compared to sulfide as sulfur donor, cysteine shows 77% of the activity compared to sulfide as sulfur donor. No correct Cys-tRNACys product is formed in presence of these sulfur donors Methanosarcina mazei ?
-
?
O-phospho-L-seryl-tRNACys + sulfide under steady-state conditions at 1-50 nM Sep-CysS, there is no reaction when O-phospho-L-seryl-tRNACys substrate levels are maintained below 0.01 mM, however, product formation is detected at 0.05 mM O-phospho-L-seryl-tRNACys Methanosarcina mazei L-cysteinyl-tRNACys + phosphate
-
?
O-phospho-L-seryl-tRNACys + sulfide SepCysS exhibits substrate preference among the three Methanosarcina mazei tRNACys isoacceptors Methanosarcina mazei L-cysteinyl-tRNACys + phosphate
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 42000, gel filtration Methanosarcina mazei
homodimer 2 * 42324, predicted from amino acid sequence Methanosarcina mazei

Synonyms

Synonyms Comment Organism
Sep-tRNA:Cys-tRNA synthase
-
Methanosarcina mazei
SepCysS
-
Methanosarcina mazei

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent Methanosarcina mazei
pyridoxal 5'-phosphate both active sites bind the cofactor Methanosarcina mazei