Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta2(DE3) pLysS cells | Methanosarcina mazei |
expression in Escherichia coli | Methanosarcina mazei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
2 * 42000, gel filtration | Methanosarcina mazei |
42324 | - |
2 * 42324, predicted from amino acid sequence | Methanosarcina mazei |
84000 | - |
gel filtration | Methanosarcina mazei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina mazei | - |
- |
- |
Methanosarcina mazei | Q8PVS9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanosarcina mazei |
DEAE column chromatography and Superdex 200 gel filtration | Methanosarcina mazei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
highly sensitive assay to measure the activity of SepCysS under anaerobic conditions | Methanosarcina mazei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | thiosulfate shows 72.6% of the activity compared to sulfide as sulfur donor, cysteine shows 77% of the activity compared to sulfide as sulfur donor. No correct Cys-tRNACys product is formed in presence of these sulfur donors | Methanosarcina mazei | ? | - |
? | |
O-phospho-L-seryl-tRNACys + sulfide | under steady-state conditions at 1-50 nM Sep-CysS, there is no reaction when O-phospho-L-seryl-tRNACys substrate levels are maintained below 0.01 mM, however, product formation is detected at 0.05 mM O-phospho-L-seryl-tRNACys | Methanosarcina mazei | L-cysteinyl-tRNACys + phosphate | - |
? | |
O-phospho-L-seryl-tRNACys + sulfide | SepCysS exhibits substrate preference among the three Methanosarcina mazei tRNACys isoacceptors | Methanosarcina mazei | L-cysteinyl-tRNACys + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 42000, gel filtration | Methanosarcina mazei |
homodimer | 2 * 42324, predicted from amino acid sequence | Methanosarcina mazei |
Synonyms | Comment | Organism |
---|---|---|
Sep-tRNA:Cys-tRNA synthase | - |
Methanosarcina mazei |
SepCysS | - |
Methanosarcina mazei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent | Methanosarcina mazei | |
pyridoxal 5'-phosphate | both active sites bind the cofactor | Methanosarcina mazei |