Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | approximately increases the activity of the enzyme 2fold and retains the effect at a similar level through various concentrations from 0.01 to 1% | Saccharolobus solfataricus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the enzyme does not show activity without a divalent cation and prefers Mg2+ to other cations such as Mn2+ and Ca2+. At concentrations ranging from 2.5 to 50 mM, the effect of Mg2+ does not change greatly | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
x * 32000, SDS-PAGE | Saccharolobus solfataricus |
32274 | - |
x * 32274, calculated from sequence | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate + 2 isopentenyl diphosphate | Saccharolobus solfataricus | - |
2 diphosphate + all-trans-hexaprenyl diphosphate | - |
? | |
geranylgeranyl diphosphate + 2 isopentenyl diphosphate | Saccharolobus solfataricus P2 | - |
2 diphosphate + all-trans-hexaprenyl diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q97W92 | - |
- |
Saccharolobus solfataricus P2 | Q97W92 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
farnesyl diphosphate + 3 isopentenyl diphosphate | the enzyme prefers geranylgeranyl diphosphate to farnesyl diphosphate as an allylic substrate and does not show activity for geranyl diphosphate and dimethylallyl diphosphate | Saccharolobus solfataricus | 3 diphosphate + all-trans-hexaprenyl diphosphate | - |
? | |
farnesyl diphosphate + 3 isopentenyl diphosphate | the enzyme prefers geranylgeranyl diphosphate to farnesyl diphosphate as an allylic substrate and does not show activity for geranyl diphosphate and dimethylallyl diphosphate | Saccharolobus solfataricus P2 | 3 diphosphate + all-trans-hexaprenyl diphosphate | - |
? | |
geranylgeranyl diphosphate + 2 isopentenyl diphosphate | - |
Saccharolobus solfataricus | 2 diphosphate + all-trans-hexaprenyl diphosphate | - |
? | |
geranylgeranyl diphosphate + 2 isopentenyl diphosphate | the enzyme prefers geranylgeranyl diphosphate to farnesyl diphosphate as an allylic substrate and does not show activity for geranyl diphosphate and dimethylallyl diphosphate. The main product is hexaprenyl diphosphate (C30) when the concentrations of isopentenyl diphosphate and geranylgeranyl diphosphate are the same. A 10-fold increase in the concentration of isopentenyl diphosphate results in the increased chain length of the products: a significant amount of heptaprenyl diphosphate (C35) is produced, although hexaprenyl diphosphate remains the main product | Saccharolobus solfataricus | 2 diphosphate + all-trans-hexaprenyl diphosphate | - |
? | |
geranylgeranyl diphosphate + 2 isopentenyl diphosphate | - |
Saccharolobus solfataricus P2 | 2 diphosphate + all-trans-hexaprenyl diphosphate | - |
? | |
geranylgeranyl diphosphate + 2 isopentenyl diphosphate | the enzyme prefers geranylgeranyl diphosphate to farnesyl diphosphate as an allylic substrate and does not show activity for geranyl diphosphate and dimethylallyl diphosphate. The main product is hexaprenyl diphosphate (C30) when the concentrations of isopentenyl diphosphate and geranylgeranyl diphosphate are the same. A 10-fold increase in the concentration of isopentenyl diphosphate results in the increased chain length of the products: a significant amount of heptaprenyl diphosphate (C35) is produced, although hexaprenyl diphosphate remains the main product | Saccharolobus solfataricus P2 | 2 diphosphate + all-trans-hexaprenyl diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, SDS-PAGE | Saccharolobus solfataricus |
? | x * 32274, calculated from sequence | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
(all-E) hexaprenyl diphosphate synthase | - |
Saccharolobus solfataricus |
(all-trans) hexaprenyl diphosphate synthase | - |
Saccharolobus solfataricus |
HexPS | - |
Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Saccharolobus solfataricus |