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Literature summary for 2.5.1.82 extracted from

  • Hemmi, H.; Ikejiri, S.; Yamashita, S.; Nishino, T.
    Novel medium-chain prenyl diphosphate synthase from the thermoacidophilic archaeon Sulfolobus solfataricus (2002), J. Bacteriol., 184, 615-620.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 approximately increases the activity of the enzyme 2fold and retains the effect at a similar level through various concentrations from 0.01 to 1% Saccharolobus solfataricus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the enzyme does not show activity without a divalent cation and prefers Mg2+ to other cations such as Mn2+ and Ca2+. At concentrations ranging from 2.5 to 50 mM, the effect of Mg2+ does not change greatly Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
x * 32000, SDS-PAGE Saccharolobus solfataricus
32274
-
x * 32274, calculated from sequence Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranylgeranyl diphosphate + 2 isopentenyl diphosphate Saccharolobus solfataricus
-
2 diphosphate + all-trans-hexaprenyl diphosphate
-
?
geranylgeranyl diphosphate + 2 isopentenyl diphosphate Saccharolobus solfataricus P2
-
2 diphosphate + all-trans-hexaprenyl diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97W92
-
-
Saccharolobus solfataricus P2 Q97W92
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
farnesyl diphosphate + 3 isopentenyl diphosphate the enzyme prefers geranylgeranyl diphosphate to farnesyl diphosphate as an allylic substrate and does not show activity for geranyl diphosphate and dimethylallyl diphosphate Saccharolobus solfataricus 3 diphosphate + all-trans-hexaprenyl diphosphate
-
?
farnesyl diphosphate + 3 isopentenyl diphosphate the enzyme prefers geranylgeranyl diphosphate to farnesyl diphosphate as an allylic substrate and does not show activity for geranyl diphosphate and dimethylallyl diphosphate Saccharolobus solfataricus P2 3 diphosphate + all-trans-hexaprenyl diphosphate
-
?
geranylgeranyl diphosphate + 2 isopentenyl diphosphate
-
Saccharolobus solfataricus 2 diphosphate + all-trans-hexaprenyl diphosphate
-
?
geranylgeranyl diphosphate + 2 isopentenyl diphosphate the enzyme prefers geranylgeranyl diphosphate to farnesyl diphosphate as an allylic substrate and does not show activity for geranyl diphosphate and dimethylallyl diphosphate. The main product is hexaprenyl diphosphate (C30) when the concentrations of isopentenyl diphosphate and geranylgeranyl diphosphate are the same. A 10-fold increase in the concentration of isopentenyl diphosphate results in the increased chain length of the products: a significant amount of heptaprenyl diphosphate (C35) is produced, although hexaprenyl diphosphate remains the main product Saccharolobus solfataricus 2 diphosphate + all-trans-hexaprenyl diphosphate
-
?
geranylgeranyl diphosphate + 2 isopentenyl diphosphate
-
Saccharolobus solfataricus P2 2 diphosphate + all-trans-hexaprenyl diphosphate
-
?
geranylgeranyl diphosphate + 2 isopentenyl diphosphate the enzyme prefers geranylgeranyl diphosphate to farnesyl diphosphate as an allylic substrate and does not show activity for geranyl diphosphate and dimethylallyl diphosphate. The main product is hexaprenyl diphosphate (C30) when the concentrations of isopentenyl diphosphate and geranylgeranyl diphosphate are the same. A 10-fold increase in the concentration of isopentenyl diphosphate results in the increased chain length of the products: a significant amount of heptaprenyl diphosphate (C35) is produced, although hexaprenyl diphosphate remains the main product Saccharolobus solfataricus P2 2 diphosphate + all-trans-hexaprenyl diphosphate
-
?

Subunits

Subunits Comment Organism
? x * 32000, SDS-PAGE Saccharolobus solfataricus
? x * 32274, calculated from sequence Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
(all-E) hexaprenyl diphosphate synthase
-
Saccharolobus solfataricus
(all-trans) hexaprenyl diphosphate synthase
-
Saccharolobus solfataricus
HexPS
-
Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Saccharolobus solfataricus