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Literature summary for 2.5.1.85 extracted from

  • Block, A.; Fristedt, R.; Rogers, S.; Kumar, J.; Barnes, B.; Barnes, J.; Elowsky, C.G.; Wamboldt, Y.; Mackenzie, S.A.; Redding, K.; Merchant, S.S.; Basset, G.J.
    Functional modeling identifies paralogous solanesyl-diphosphate synthases that assemble the side chain of plastoquinone-9 in plastids (2013), J. Biol. Chem., 288, 27594-27606.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
gene AtSPS1, phylogenetic analysis, recombinant expression of N-terminally GST-tagged enzyme in Nicotiana benthamiana epidermis cell endoplasmic reticulum using Agrobacterium tumefaciens strain C58C1 for transfection Arabidopsis thaliana
gene AtSPS2, phylogenetic analysis, recombinant expression of N-terminally GST-tagged enzyme in Nicotiana benthamiana leaf chloroplasts using Agrobacterium tumefaciens strain C58C1 for transfection Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information construction of a T-DNA insertion mutant SALK_064292 from gene At1g17050 encoding AtSPS2 Arabidopsis thaliana
additional information construction of a T-DNA insertion mutant SALK_126948 from gene At1g78510 encoding AtSPS1 Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Arabidopsis thaliana 9507
-
endoplasmic reticulum
-
 Arabidopsis thaliana 5783
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranylgeranyl diphosphate + 5 isopentenyl diphosphate Arabidopsis thaliana
-
 5 diphosphate + all-trans-nonaprenyl diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q76FS5 gene AtSPS2
-
Arabidopsis thaliana Q8S948 gene AtSPS1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranylgeranyl diphosphate + 5 isopentenyl diphosphate
-
 Arabidopsis thaliana 5 diphosphate + all-trans-nonaprenyl diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
AtSPS1
-
 Arabidopsis thaliana
AtSPS2
-
 Arabidopsis thaliana
solanesyl-diphosphate synthase
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
evolution co-expression network of the Arabidopsis thaliana solanesyl-diphosphate synthase family Arabidopsis thaliana
malfunction in contrast to other plastochromanol-8 biosynthetic mutants, neither the single atsps knock-outs nor the atsps1 atsps2 double knock-out display any defects in tocopherols accumulation or germination Arabidopsis thaliana
malfunction leaves of the atsps2 knock-out are devoid of plastochromanol-8 and display severe losses of both non-photoactive and photoactive plastoquinone-9, resulting in near complete photoinhibition at high light intensity. The photoinhibition is paralleled by significant damage to photosystem II but not to photosystem I. In contrast to other plastochromanol-8 biosynthetic mutants, neither the single atsps knock-outs nor the atsps1 atsps2 double knock-out display any defects in tocopherols accumulation or germination Arabidopsis thaliana
physiological function plastid isoforms of solanesyl-diphosphate synthase catalyze the elongation of the prenyl side chain involved in the plastoquinone-9 and plastochromanol-8 biosynthesis, plastochromanol-8 originates from a subfraction of the non-photoactive pool of plastoquinone-9 Arabidopsis thaliana