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Literature summary for 2.5.1.87 extracted from
- Yeast Saccharomyces cerevisiae has two cis-prenyltransferases with different properties and localizations. Implication for their distinct physiological roles in dolichol synthesis (2001), Genes Cells, 6, 495-506.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| over-expression of SRT1 suppresses the growth and glycosylation defects of rer2 | Saccharomyces cerevisiae |
| when GFP-RER2 is over-expressed under the TDH3 promoter in DELTArer2, a continuous endioplasmic reticulum pattern and some dots associated with the endoplasmic reticulum are observed in the early logarithmic phase | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | localized to the continuous endoplasmic reticulum and some dots associated with the endoplasmic reticulum | Saccharomyces cerevisiae | 5783 | - |
| lipid particle | Srt1p resides mostly in lipid particles | Saccharomyces cerevisiae | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate + 13 isopentenyl diphosphate | Saccharomyces cerevisiae | - |
13 diphosphate + di-trans,poly-cis-hexadecaprenyl diphosphate | di-trans,poly-cis-hexadecaprenyl diphosphate and di-trans,poly-cis-heptadecaprenyl diphosphate are the main products | ? |  |
| (2E,6E)-farnesyl diphosphate + 14 isopentenyl diphosphate | Saccharomyces cerevisiae | - |
14 diphosphate + di-trans,poly-cis-heptadecaprenyl diphosphate | di-trans,poly-cis-hexadecaprenyl diphosphate and di-trans,poly-cis-heptadecaprenyl diphosphate are the main products | ? | |
| (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | Saccharomyces cerevisiae | the polyprenol product of Srt1p is longer in chain length than that of Rer2p and is not sufficiently converted to dolichol and dolichyl phosphate, unlike that of Rer2p | 5 diphosphate + di-trans,poly-cis-octaprenyl diphosphate | di-trans,poly-cis-octaprenyl diphosphate and di-trans,poly-cis-nonaprenyl diphosphate are the main products | ? | |
| (2E,6E)-farnesyl diphosphate + 6 isopentenyl diphosphate | Saccharomyces cerevisiae | the polyprenol product of Srt1p is longer in chain length than that of Rer2p and is not sufficiently converted to dolichol and dolichyl phosphate, unlike that of Rer2p | 6 diphosphate + di-trans,poly-cis-nonaprenyl diphosphate | di-trans,poly-cis-octaprenyl diphosphate and di-trans,poly-cis-nonaprenyl diphosphate are the main products | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | C7GM47 | - |
- |
| Saccharomyces cerevisiae | P35196 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate + 13 isopentenyl diphosphate | - |
Saccharomyces cerevisiae | 13 diphosphate + di-trans,poly-cis-hexadecaprenyl diphosphate | di-trans,poly-cis-hexadecaprenyl diphosphate and di-trans,poly-cis-heptadecaprenyl diphosphate are the main products | ? | |
| (2E,6E)-farnesyl diphosphate + 14 isopentenyl diphosphate | - |
Saccharomyces cerevisiae | 14 diphosphate + di-trans,poly-cis-heptadecaprenyl diphosphate | di-trans,poly-cis-hexadecaprenyl diphosphate and di-trans,poly-cis-heptadecaprenyl diphosphate are the main products | ? | |
| (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | - |
Saccharomyces cerevisiae | 5 diphosphate + di-trans,poly-cis-octaprenyl diphosphate | di-trans,poly-cis-octaprenyl diphosphate and di-trans,poly-cis-nonaprenyl diphosphate are the main products | ? | |
| (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | the polyprenol product of Srt1p is longer in chain length than that of Rer2p and is not sufficiently converted to dolichol and dolichyl phosphate, unlike that of Rer2p | Saccharomyces cerevisiae | 5 diphosphate + di-trans,poly-cis-octaprenyl diphosphate | di-trans,poly-cis-octaprenyl diphosphate and di-trans,poly-cis-nonaprenyl diphosphate are the main products | ? | |
| (2E,6E)-farnesyl diphosphate + 6 isopentenyl diphosphate | - |
Saccharomyces cerevisiae | 6 diphosphate + di-trans,poly-cis-nonaprenyl diphosphate | di-trans,poly-cis-octaprenyl diphosphate and di-trans,poly-cis-nonaprenyl diphosphate are the main products | ? | |
| (2E,6E)-farnesyl diphosphate + 6 isopentenyl diphosphate | the polyprenol product of Srt1p is longer in chain length than that of Rer2p and is not sufficiently converted to dolichol and dolichyl phosphate, unlike that of Rer2p | Saccharomyces cerevisiae | 6 diphosphate + di-trans,poly-cis-nonaprenyl diphosphate | di-trans,poly-cis-octaprenyl diphosphate and di-trans,poly-cis-nonaprenyl diphosphate are the main products | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cis-prenyltransferase | - |
Saccharomyces cerevisiae |
| RER2 | - |
Saccharomyces cerevisiae |
| SRT1 | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Saccharomyces cerevisiae |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Saccharomyces cerevisiae | induced in the stationary phase | up |
| Saccharomyces cerevisiae | mainly expressed in the early logarithmic phase | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | involved in biosynthesis of dolichol (a long-chain polyprenol with a saturated alpha-isoprene unit, which serves as a glycosyl carrier in protein glycosylation). Of the two isozymes, Rer2p and Srt1p, isoenzyme Rer2p is responsible for the main activity of the wild-type cells, and the expression of RER2 is highest in the early logarithmic phase when the cells are actively dividing | Saccharomyces cerevisiae |
| physiological function | over-expression of SRT1 suppresses the growth and glycosylation defects of rer2. SRT1 has only a minor contribution to total cis-prenyltransferase activity under normal conditions | Saccharomyces cerevisiae |
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