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Literature summary for 2.5.1.9 extracted from

  • Bacher, A.; Baur, R.; Eggers, U.; Harders, H.D.; Otto, M.K.; Schnepple, H.
    Riboflavin synthases of Bacillus subtilis. Purification and properties (1980), J. Biol. Chem., 255, 632-637.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
SO32- enhances activity Bacillus subtilis
SO32- enhances activity Saccharomyces cerevisiae
SO32- sodium sulfite Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
6,7-dimethyl-8-(1'-D-ribityl)lumazine heavy enzyme Bacillus subtilis
0.014
-
6,7-dimethyl-8-(1'-D-ribityl)lumazine light enzyme Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23500
-
3 * 23500, light enzyme, SDS-PAGE, subunits not covalently linked Bacillus subtilis
70000
-
analytical ultracentrifugation Bacillus subtilis
70000
-
light enzyme Bacillus subtilis
100000
-
analytical ultracentrifugation Bacillus subtilis
100000
-
heavy riboflavin synthase Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Lactiplantibacillus plantarum
-
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Bacillus subtilis
-
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Escherichia coli
-
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Saccharomyces cerevisiae
-
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Priestia megaterium
-
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Moorella thermoacetica
-
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Bacillus licheniformis
-
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Paenibacillus polymyxa
-
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Streptomyces venezuelae
-
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Rhodococcus ruber
-
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Pseudomonas iodinum
-
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Escherichia coli B / ATCC 11303
-
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis
-
contains heavy and light form of the enzyme
-
Bacillus subtilis
-
heavy and light enzyme form
-
Bacillus subtilis
-
H94) (flavinogenic), H322 (flavinogenic), (H52, Rib-), (107,Rib-), ATCC 6051, ATCC 6633, B23
-
Bacillus subtilis
-
contains heavy and light form of the enzyme
-
Escherichia coli
-
B
-
Escherichia coli B / ATCC 11303
-
B
-
Lactiplantibacillus plantarum
-
-
-
Moorella thermoacetica
-
contains heavy and light form of the enzyme
-
Paenibacillus polymyxa
-
contains heavy and light form of the enzyme
-
Priestia megaterium
-
contains heavy and light form of the enzyme
-
Pseudomonas iodinum
-
-
-
Rhodococcus ruber
-
-
-
Saccharomyces cerevisiae
-
-
-
Streptomyces venezuelae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
heavy enzyme Bacillus subtilis
light enzyme Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.033
-
heavy enzyme Bacillus subtilis
0.833
-
light enzyme Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
Lactiplantibacillus plantarum riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
Bacillus subtilis riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
Escherichia coli riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
Saccharomyces cerevisiae riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
Priestia megaterium riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
Moorella thermoacetica riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
Bacillus licheniformis riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
Paenibacillus polymyxa riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
Streptomyces venezuelae riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
Rhodococcus ruber riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
Pseudomonas iodinum riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
Escherichia coli B / ATCC 11303 riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?

Subunits

Subunits Comment Organism
trimer
-
Escherichia coli
trimer 3 * 23500, light enzyme, SDS-PAGE, subunits not covalently linked Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2
-
6,7-dimethyl-8-(1'-D-ribityl)lumazine heavy enzyme, Bacillus subtilis H94 Bacillus subtilis
2
-
6,7-dimethyl-8-(1'-D-ribityl)lumazine light enzyme, Bacillus subtilis H94 Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
light enzyme, the pH-optimum of the heavy enzyme is similar, detailed measurement is not possible because of instability at elevated pH Bacillus subtilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
heavy enzyme is instable at elevated pH Bacillus subtilis