Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for a cofactor | Geobacillus stearothermophilus |
General Stability | Organism |
---|---|
stable at 26°C in 4 M urea for 18 h, 4 M urea completely inactivates at 55°C | Geobacillus stearothermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | - |
Geobacillus stearothermophilus | |
6-Methyl-7-hydroxy-8-ribityllumazine | - |
Geobacillus stearothermophilus | |
Ca2+ | - |
Geobacillus stearothermophilus | |
Cu2+ | - |
Geobacillus stearothermophilus | |
Hg2+ | - |
Geobacillus stearothermophilus | |
iodoacetamide | - |
Geobacillus stearothermophilus | |
Mg2+ | - |
Geobacillus stearothermophilus | |
Mn2+ | - |
Geobacillus stearothermophilus | |
additional information | no effect by Ba2+, Co2+, EDTA | Geobacillus stearothermophilus | |
p-chloromercuribenzoate | - |
Geobacillus stearothermophilus | |
Zn2+ | - |
Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km is temperature-dependent | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect by Ba2+, Co2+ | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
345000 | - |
gel filtration | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine | Geobacillus stearothermophilus | - |
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
strain ATCC 8005 | - |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.011 | - |
purified enzyme | Geobacillus stearothermophilus |
Storage Stability | Organism |
---|---|
4°C, 6 months, no loss of activity | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine | - |
Geobacillus stearothermophilus | riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
- |
Geobacillus stearothermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 95 | 50°C: about 10% of activity maximum, 80°C: about 30% of activity maximum, 95°C: activity maximum | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
24 h, no loss of activity, without substrate | Geobacillus stearothermophilus |
85 | - |
10 min, complete loss of activity | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | 7.2 | - |
Geobacillus stearothermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.4 | 8.8 | about 50% of activity maximum at pH 5.4 and 8.8 | Geobacillus stearothermophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | 26°C, 18 h, stable | Geobacillus stearothermophilus |
6.3 | 7.6 | 55°C, 18 h, stable | Geobacillus stearothermophilus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
6-Methyl-7-hydroxy-8-ribityllumazine | - |
Geobacillus stearothermophilus |