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Literature summary for 2.5.1.91 extracted from

  • Zahiri, H.S.; Noghabi, K.A.; Shin, Y.C.
    Biochemical characterization of the decaprenyl diphosphate synthase of Rhodobacter sphaeroides for coenzyme Q10 production (2006), Appl. Microbiol. Biotechnol., 73, 796-806.
    View publication on PubMed
Search for more literature for 2.5.1.91

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 activates, 0.3% provides maximum activity Cereibacter sphaeroides
Triton X-100 the addition of Triton X-100, even at the lowest tested concentration of 0.05%, results in a significant increase in enzyme activity. Triton X-100 at 0.3% provides the maximumactivity Cereibacter sphaeroides

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Cereibacter sphaeroides
expression in Escherichia coli. Wild-type Escherichia coli produces coenzyme Q8 and does not produce any coenzyme Q10. Recombinant Escherichia coli produces coenzyme Q10 in addition to coenzyme Q8 Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0115
-
 (2E,6E,10E)-geranylgeranyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides
0.0115
-
 geranyl geranyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides
0.0131
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides
0.0138
-
 isopentenyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides
0.0185
-
 geranyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 50% increase in activity in presence of KCl Cereibacter sphaeroides
KCl removal of KCl from the reaction mixture results in a decrease of activity Cereibacter sphaeroides
Mg2+ absolutely required for activity, maximum activity at 5 mM Cereibacter sphaeroides
MgCl2 essential for activity, highest enzyme activity with 5 mM MgCl2 Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38100
-
 x * 38100, calculated from sequence Cereibacter sphaeroides
38100
-
 x * 38100, SDS-PAGE and calculated Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides Q5I7E1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate best substrate Cereibacter sphaeroides 7 diphosphate + all-trans-decaprenyl diphosphate high product specificity for decaprenyl synthesis ?
(2E,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate compared with other priming substrates examined (geranyl diphosphate, geranylgeranyl diphosphate), farnesyl diphosphate results in the highest values for the reaction rate and kcat/KM. Dimethylallyl diphosphate is not a suitable substrate Cereibacter sphaeroides 7 diphosphate + all-trans-decaprenyl diphosphate decaprenyl diphosphate is the major product, other prenyl diphosphates comprise only minor components ?
(2E,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate
-
 Cereibacter sphaeroides diphosphate + ?
-
 ?
dimethyl allyl diphosphate + isopentenyl diphosphate
-
 Cereibacter sphaeroides diphosphate + ?
-
 ?
geranyl diphosphate + 8 isopentenyl diphosphate
-
 Cereibacter sphaeroides 8 diphosphate + all-trans-decaprenyl diphosphate decaprenyl diphosphate is the major product, other prenyl diphosphates comprise only minor components ?
geranyl diphosphate + isopentenyl diphosphate
-
 Cereibacter sphaeroides diphosphate + ?
-
 ?
geranylgeranyl diphosphate + 6 isopentenyl diphosphate
-
 Cereibacter sphaeroides 6 diphosphate + all-trans-decaprenyl diphosphate decaprenyl diphosphate is the major product, other prenyl diphosphates comprise only minor components ?
additional information catalyzes the addition of isopentenyl diphosphate to dimethyl allyl diphosphate, geranyl diphosphate, (2E,6E)-farnesyl diphosphate, and (2E,6E,10E)-geranylgeranyl diphosphate as priming substrates Cereibacter sphaeroides ?
-
 ?

Subunits

Subunits Comment Organism
? x * 38100, calculated from sequence Cereibacter sphaeroides
? x * 38100, SDS-PAGE and calculated Cereibacter sphaeroides

Synonyms

Synonyms Comment Organism
Ddps
-
 Cereibacter sphaeroides
Rsdds
-
 Cereibacter sphaeroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Cereibacter sphaeroides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.058
-
 (2E,6E,10E)-geranylgeranyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides
0.06
-
 geranyl geranyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides
0.067
-
 geranyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides
0.14
-
 isopentenyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides
0.143
-
 isopentenyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides
0.147
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Cereibacter sphaeroides

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.62
-
 geranyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides
5.04
-
 (2E,6E,10E)-geranylgeranyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides
5.2
-
 geranyl geranyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides
10.36
-
 isopentenyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides
10.77
-
 isopentenyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides
11.2
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides
11.22
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides