Crystallization (Comment) | Organism |
---|---|
crystals of cytosolic enzyme, or enzyme in complex with substrates, N-methylpyridoxal, and inhibitors, depression-plate and sandwich-box techniques, protein solution: 35-40 mg/ml, pH 5.4, 40 mM sodium acetate, 4% polyethylene glycol 4000-6000, with or without substrate, cofactor or inhibitor compounds, reservoir solution: 40 mM sodium acetate, pH 5.4, 8% polyethylene glycol 4000-6000, crystals appear within 1 week, spectroscopic analysis | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | cytosolic isoenzyme | Sus scrofa | 5829 | - |
mitochondrion | mitochondrial isoenzyme | Sus scrofa | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | Sus scrofa | - |
oxaloacetate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate | bi bi ping pong reaction kinetic | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | - |
Sus scrofa | oxaloacetate + L-glutamate | - |
? |