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Literature summary for 2.6.1.1 extracted from

  • Metzler, C.M.; Rogers, P.H.; Arone, A.; Martin, D.S.; Metzler, D.E.
    Investigation of crystalline enzyme-substrate complexes of pyridoxal phosphate-dependent enzymes (1979), Methods Enzymol., 62, 551-558.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals of cytosolic enzyme, or enzyme in complex with substrates, N-methylpyridoxal, and inhibitors, depression-plate and sandwich-box techniques, protein solution: 35-40 mg/ml, pH 5.4, 40 mM sodium acetate, 4% polyethylene glycol 4000-6000, with or without substrate, cofactor or inhibitor compounds, reservoir solution: 40 mM sodium acetate, pH 5.4, 8% polyethylene glycol 4000-6000, crystals appear within 1 week, spectroscopic analysis Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol cytosolic isoenzyme Sus scrofa 5829
-
mitochondrion mitochondrial isoenzyme Sus scrofa 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate + 2-oxoglutarate Sus scrofa
-
oxaloacetate + L-glutamate
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Reaction

Reaction Comment Organism Reaction ID
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate bi bi ping pong reaction kinetic Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + 2-oxoglutarate
-
Sus scrofa oxaloacetate + L-glutamate
-
?