Literature summary for 2.6.1.1 extracted from

Kamitori, S.; Hirotsu, K.; Higuchi, T.; Kondo, K.; Inoue, K.; Kuramitsu, S.; Kagamiyama, H.; Higuchi, Y.; Yasuoka, N.; Kusunoki, M.; Matsuura, Y.
Overproduction and preliminary X-ray characterization of aspartate aminotransferase from Escherichia coli (1987), J. Biochem., 101, 813-816.

Search for more literature for 2.6.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression from plasmid in strain JM103	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop method, protein solution: 30 mg/ml, 10 mM potassium phosphate, pH 7.0, 0.01 mM pyridoxal 5'-phosphate, 1 mM EDTA, 2 mM succinate, 0.3 mM azide, reservoir solution: same composition as protein solution, contains no protein but polyethylene glycol 4000, 11-17% w/v, 20°C, stepwise addition of protein solution, X-ray diffraction analysis	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-aspartate + 2-oxoglutarate	Escherichia coli	-	oxaloacetate + L-glutamate	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinantly overexpressed enzyme	Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
232	-	purified recombinant enyme	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-aspartate + 2-oxoglutarate	-	Escherichia coli	oxaloacetate + L-glutamate	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 42000-45000, SDS-PAGE	Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Escherichia coli

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)