Crystallization (Comment) | Organism |
---|---|
purified mutant K258A, batch method, protein solution: 5.2 mg/ml, 50 mM potassium phosphate, pH 7.5, 53% ammonium sulfate, room temperature, 1 week, preparation of crystals with heavy atom derivatives by soaking crystals for 24 or 48 h, structure analysis | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
K258A | exchange of active site lysine for alanine, binds still pyridoxal 5'-phosphate or pyridoxamine 5'-phosphate, but not covalently, the latter forms when bound to the enzyme a covalent stable ketimine with 2-oxoglutarate and effects a beta-decarboxylation of oxaloacetate, followed by transamination to give the pyridoxal 5'-phosphate aldimine of alanine as a final product | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | Escherichia coli | - |
oxaloacetate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
mutant K258A | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate | mechanism | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | - |
Escherichia coli | oxaloacetate + L-glutamate | - |
? | |
L-aspartate + 2-oxoglutarate | - |
Escherichia coli | oxaloacetate + L-glutamate | - |
r |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | a pyridoxal 5'-phosphate protein | Escherichia coli | |
pyridoxamine 5'-phosphate | reverse reaction | Escherichia coli |