Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.6.1.1 extracted from

  • Tanaka, T.; Yamamoto, S.; Taniguchi, M.; Hayashi, H.; Kuramitsu, S.; Kagamiyama, H.; Oi, S.
    Further studies on aspartate aminotransferase of thermophilic methanogens by analysis of general properties, bound cofactors, and subunit structures (1992), J. Biochem., 112, 811-815.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
4 * 43000, SDS-PAGE Methanothermobacter thermautotrophicus
180000
-
gel filtration Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate + 2-oxoglutarate Methanothermobacter thermautotrophicus
-
oxaloacetate + L-glutamate
-
?
L-aspartate + 2-oxoglutarate Methanothermobacter thermautotrophicus SF-4
-
oxaloacetate + L-glutamate
-
?

Organism

Organism UniProt Comment Textmining
Methanothermobacter thermautotrophicus
-
-
-
Methanothermobacter thermautotrophicus
-
strains FTF-INRA, DSM 3012
-
Methanothermobacter thermautotrophicus
-
DSM 6457
-
Methanothermobacter thermautotrophicus SF-4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanothermobacter thermautotrophicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
21.8
-
purified enzyme Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + 2-oxoglutarate
-
Methanothermobacter thermautotrophicus oxaloacetate + L-glutamate
-
?
L-aspartate + 2-oxoglutarate
-
Methanothermobacter thermautotrophicus SF-4 oxaloacetate + L-glutamate
-
?
L-cysteine + 2-oxoglutarate
-
Methanothermobacter thermautotrophicus 3-mercaptopyruvate + L-glutamate
-
?
L-cysteine + 2-oxoglutarate
-
Methanothermobacter thermautotrophicus SF-4 3-mercaptopyruvate + L-glutamate
-
?
L-glutamate + 2-oxoglutarate
-
Methanothermobacter thermautotrophicus 2-oxoglutarate + L-glutamate
-
?
L-glutamate + 2-oxoglutarate
-
Methanothermobacter thermautotrophicus SF-4 2-oxoglutarate + L-glutamate
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 43000, SDS-PAGE Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Methanothermobacter thermautotrophicus
70
-
strain FTF-INRA Methanothermobacter thermautotrophicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
pH 7.5, 1 h, gradual loss of activity above Methanothermobacter thermautotrophicus
70
-
pH 7.5, 1 h, stable up to Methanothermobacter thermautotrophicus
70
-
pH 7.5, 1 h, enzyme loses 65% of activity Methanothermobacter thermautotrophicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Methanothermobacter thermautotrophicus