Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
N165Y/R316H/A381T/P399L | the mutant shows increased activity compared to the wild type enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.92 | - |
phenylpyruvate | mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C | Escherichia coli | |
1.01 | - |
3,4-dimethoxyphenylpyruvate | mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C | Escherichia coli | |
4.12 | - |
4-methoxyphenylpyruvate | mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C | Escherichia coli | |
4.24 | - |
phenylpyruvate | wild type enzyme, at pH 8.0 and 40°C | Escherichia coli | |
4.92 | - |
3-methoxyphenylpyruvate | mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C | Escherichia coli | |
5.84 | - |
2-methoxyphenylpyruvate | mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C | Escherichia coli | |
10.14 | - |
4-methoxyphenylpyruvate | wild type enzyme, at pH 8.0 and 40°C | Escherichia coli | |
10.32 | - |
3,4-dimethoxyphenylpyruvate | wild type enzyme, at pH 8.0 and 40°C | Escherichia coli | |
11.56 | - |
3-methoxyphenylpyruvate | wild type enzyme, at pH 8.0 and 40°C | Escherichia coli | |
12.07 | - |
2-methoxyphenylpyruvate | wild type enzyme, at pH 8.0 and 40°C | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | Escherichia coli | - |
oxaloacetate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-methoxyphenylpyruvate | 67.8% conversion | Escherichia coli | oxaloacetate + ? | - |
? | |
L-aspartate + 2-oxoglutarate | - |
Escherichia coli | oxaloacetate + L-glutamate | - |
? | |
L-aspartate + 3,4-dimethoxyphenylpyruvate | 72.5% conversion | Escherichia coli | oxaloacetate + L-3,4-dimethoxyphenylalanine | - |
? | |
L-aspartate + 3-methoxyphenylpyruvate | 70.2% conversion | Escherichia coli | oxaloacetate + ? | - |
? | |
L-aspartate + 4-methoxyphenylpyruvate | 73.6% conversion | Escherichia coli | oxaloacetate + ? | - |
? | |
L-aspartate + phenylpyruvate | 83.4% conversion | Escherichia coli | oxaloacetate + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aspartate aminotransferase | - |
Escherichia coli |
AspAT | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.44 | - |
2-methoxyphenylpyruvate | wild type enzyme, at pH 8.0 and 40°C | Escherichia coli | |
1.55 | - |
3-methoxyphenylpyruvate | wild type enzyme, at pH 8.0 and 40°C | Escherichia coli | |
1.77 | - |
4-methoxyphenylpyruvate | wild type enzyme, at pH 8.0 and 40°C | Escherichia coli | |
1.81 | - |
3,4-dimethoxyphenylpyruvate | wild type enzyme, at pH 8.0 and 40°C | Escherichia coli | |
2.69 | - |
2-methoxyphenylpyruvate | mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C | Escherichia coli | |
2.79 | - |
3-methoxyphenylpyruvate | mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C | Escherichia coli | |
2.85 | - |
4-methoxyphenylpyruvate | mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C | Escherichia coli | |
2.99 | - |
phenylpyruvate | wild type enzyme, at pH 8.0 and 40°C | Escherichia coli | |
3.45 | - |
phenylpyruvate | mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C | Escherichia coli | |
4.33 | - |
3,4-dimethoxyphenylpyruvate | mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.119 | - |
2-methoxyphenylpyruvate | wild type enzyme, at pH 8.0 and 40°C | Escherichia coli | |
0.134 | - |
3-methoxyphenylpyruvate | wild type enzyme, at pH 8.0 and 40°C | Escherichia coli | |
0.175 | - |
4-methoxyphenylpyruvate | wild type enzyme, at pH 8.0 and 40°C | Escherichia coli | |
0.175 | - |
3,4-dimethoxyphenylpyruvate | wild type enzyme, at pH 8.0 and 40°C | Escherichia coli | |
0.461 | - |
2-methoxyphenylpyruvate | mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C | Escherichia coli | |
0.567 | - |
3-methoxyphenylpyruvate | mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C | Escherichia coli | |
0.692 | - |
4-methoxyphenylpyruvate | mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C | Escherichia coli | |
0.705 | - |
phenylpyruvate | wild type enzyme, at pH 8.0 and 40°C | Escherichia coli | |
3.75 | - |
phenylpyruvate | mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C | Escherichia coli | |
4.287 | - |
3,4-dimethoxyphenylpyruvate | mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C | Escherichia coli |