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Literature summary for 2.6.1.1 extracted from

  • Yu, J.; Li, J.; Cao, S.; Wu, T.; Zeng, S.; Zhang, H.; Liu, J.; Jiao, Q.
    Chemoenzymatic synthesis of L-3,4-dimethoxyphenyl-alanine and its analogues using aspartate aminotransferase as a key catalyst (2019), Catal. Commun., 120, 28-32 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
N165Y/R316H/A381T/P399L the mutant shows increased activity compared to the wild type enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.92
-
phenylpyruvate mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C Escherichia coli
1.01
-
3,4-dimethoxyphenylpyruvate mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C Escherichia coli
4.12
-
4-methoxyphenylpyruvate mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C Escherichia coli
4.24
-
phenylpyruvate wild type enzyme, at pH 8.0 and 40°C Escherichia coli
4.92
-
3-methoxyphenylpyruvate mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C Escherichia coli
5.84
-
2-methoxyphenylpyruvate mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C Escherichia coli
10.14
-
4-methoxyphenylpyruvate wild type enzyme, at pH 8.0 and 40°C Escherichia coli
10.32
-
3,4-dimethoxyphenylpyruvate wild type enzyme, at pH 8.0 and 40°C Escherichia coli
11.56
-
3-methoxyphenylpyruvate wild type enzyme, at pH 8.0 and 40°C Escherichia coli
12.07
-
2-methoxyphenylpyruvate wild type enzyme, at pH 8.0 and 40°C Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate + 2-oxoglutarate Escherichia coli
-
oxaloacetate + L-glutamate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + 2-methoxyphenylpyruvate 67.8% conversion Escherichia coli oxaloacetate + ?
-
?
L-aspartate + 2-oxoglutarate
-
Escherichia coli oxaloacetate + L-glutamate
-
?
L-aspartate + 3,4-dimethoxyphenylpyruvate 72.5% conversion Escherichia coli oxaloacetate + L-3,4-dimethoxyphenylalanine
-
?
L-aspartate + 3-methoxyphenylpyruvate 70.2% conversion Escherichia coli oxaloacetate + ?
-
?
L-aspartate + 4-methoxyphenylpyruvate 73.6% conversion Escherichia coli oxaloacetate + ?
-
?
L-aspartate + phenylpyruvate 83.4% conversion Escherichia coli oxaloacetate + ?
-
?

Synonyms

Synonyms Comment Organism
aspartate aminotransferase
-
Escherichia coli
AspAT
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.44
-
2-methoxyphenylpyruvate wild type enzyme, at pH 8.0 and 40°C Escherichia coli
1.55
-
3-methoxyphenylpyruvate wild type enzyme, at pH 8.0 and 40°C Escherichia coli
1.77
-
4-methoxyphenylpyruvate wild type enzyme, at pH 8.0 and 40°C Escherichia coli
1.81
-
3,4-dimethoxyphenylpyruvate wild type enzyme, at pH 8.0 and 40°C Escherichia coli
2.69
-
2-methoxyphenylpyruvate mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C Escherichia coli
2.79
-
3-methoxyphenylpyruvate mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C Escherichia coli
2.85
-
4-methoxyphenylpyruvate mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C Escherichia coli
2.99
-
phenylpyruvate wild type enzyme, at pH 8.0 and 40°C Escherichia coli
3.45
-
phenylpyruvate mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C Escherichia coli
4.33
-
3,4-dimethoxyphenylpyruvate mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.119
-
2-methoxyphenylpyruvate wild type enzyme, at pH 8.0 and 40°C Escherichia coli
0.134
-
3-methoxyphenylpyruvate wild type enzyme, at pH 8.0 and 40°C Escherichia coli
0.175
-
4-methoxyphenylpyruvate wild type enzyme, at pH 8.0 and 40°C Escherichia coli
0.175
-
3,4-dimethoxyphenylpyruvate wild type enzyme, at pH 8.0 and 40°C Escherichia coli
0.461
-
2-methoxyphenylpyruvate mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C Escherichia coli
0.567
-
3-methoxyphenylpyruvate mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C Escherichia coli
0.692
-
4-methoxyphenylpyruvate mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C Escherichia coli
0.705
-
phenylpyruvate wild type enzyme, at pH 8.0 and 40°C Escherichia coli
3.75
-
phenylpyruvate mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C Escherichia coli
4.287
-
3,4-dimethoxyphenylpyruvate mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C Escherichia coli