Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 CodonPlus (DE3)-RIL cells | Geobacillus sp. MAS1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not influenced by EDTA | Geobacillus sp. MAS1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
L-aspartate | at pH 8.0 and 60°C | Geobacillus sp. MAS1 | |
2.5 | - |
2-oxoglutarate | using L-aspartate as a cosubstrate, at pH 8.0 and 60°C | Geobacillus sp. MAS1 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is independent from metal ions | Geobacillus sp. MAS1 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
gel filtration | Geobacillus sp. MAS1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | Geobacillus sp. MAS1 | - |
oxaloacetate + L-glutamate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus sp. MAS1 | A0A6F8T0V6 | - |
- |
Purification (Comment) | Organism |
---|---|
HiTrap QFF column chromatography, HiTrap SPFF column chromatography and Superdex 200X gel filtration | Geobacillus sp. MAS1 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11 | - |
crude extract, at pH 8.0 and 60°C | Geobacillus sp. MAS1 |
104 | - |
after 9.5fold purification, at pH 8.0 and 60°C | Geobacillus sp. MAS1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | - |
Geobacillus sp. MAS1 | oxaloacetate + L-glutamate | - |
r | |
L-aspartate + 2-oxoglutarate | 100% activity | Geobacillus sp. MAS1 | oxaloacetate + L-glutamate | - |
r | |
L-proline + 2-oxoglutarate | 16% activity compared to L-aspartate | Geobacillus sp. MAS1 | ? | - |
? | |
L-tyrosine + 2-oxoglutarate | 27% activity compared to L-aspartate | Geobacillus sp. MAS1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 42295, MALDI-TOF mass spectrometry | Geobacillus sp. MAS1 |
homodimer | 2 * 42562, calculated from amino acid sequence | Geobacillus sp. MAS1 |
Synonyms | Comment | Organism |
---|---|---|
aspartate aminotransferase | - |
Geobacillus sp. MAS1 |
AST | - |
Geobacillus sp. MAS1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Geobacillus sp. MAS1 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 75 | more than 60% activity between 50 and 75°C | Geobacillus sp. MAS1 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 75 | there is no significant change in activity after 16 h incubation at 65°C. At 75°C the stability is reduced drastically and the half-life of the enzyme is reduced to 15 min | Geobacillus sp. MAS1 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
36.83 | - |
2-oxoglutarate | using L-aspartate as a cosubstrate, at pH 8.0 and 60°C | Geobacillus sp. MAS1 | |
4170 | - |
L-aspartate | at pH 8.0 and 60°C | Geobacillus sp. MAS1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Geobacillus sp. MAS1 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | more than 50% activity at pH 6.0-8.5 | Geobacillus sp. MAS1 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Geobacillus sp. MAS1 |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Geobacillus sp. MAS1 | calculated from amino acid sequence | - |
5.5 |