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Literature summary for 2.6.1.110 extracted from

  • Hong, L.; Zhao, Z.; Melancon III, C.; Zhang, H.; Liu, H.
    In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa (2008), J. Am. Chem. Soc., 130, 4954-4967.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.110

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Saccharopolyspora spinosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 2 * 43000, SDS-PAGE Saccharopolyspora spinosa
43356
-
 2 * 43356, calculated from amino acid sequence Saccharopolyspora spinosa
85900
-
 gel filtration Saccharopolyspora spinosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
TDP-4-dehydro-2,3,6-trideoxy-D-glucose + 2-oxoglutarate Saccharopolyspora spinosa
-
 TDP-4-amino-2,3,4,6-tetradeoxy-D-glucose + L-glutamate
-
 r

Organism

Organism UniProt Comment Textmining
Saccharopolyspora spinosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Sephacryl S-200 gel filtration Saccharopolyspora spinosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTDP-2,6-dideoxy-alpha-D-threo-hexopyranos-4-ulose + L-glutamate in the presence of pyridoxal 5'-phosphate Saccharopolyspora spinosa dTDP-4-amino-2,4,6-trideoxy-alpha-D-arabino-hexopyranose + 2-oxoglutarate
-
 ir
dTMP-5-amino-2,6-anhydro-1,3,4,5,7-pentadeoxy-1-phosphono-D-arabino-heptitol + 2-oxoglutarate in the presence of pyridoxal 5'-phosphate Saccharopolyspora spinosa dTMP-2,6-anhydro-5-dehydro-1,3,4,7-tetradeoxy-1-phosphono-D-arabino-heptitol + L-glutamate
-
 r
TDP-4-dehydro-2,3,6-trideoxy-D-glucose + 2-oxoglutarate
-
 Saccharopolyspora spinosa TDP-4-amino-2,3,4,6-tetradeoxy-D-glucose + L-glutamate
-
 r
TDP-4-dehydro-2,6-dideoxy-D-glucose + 2-oxoglutarate
-
 Saccharopolyspora spinosa TDP-4-amino-2,4,6-trideoxy-D-glucose + L-glutamate
-
 r
TDP-C-4-amino-2,3,4,6-tetradeoxy-alpha-D-erythro-hexopyranose + 2-oxoglutarate
-
 Saccharopolyspora spinosa ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 43000, SDS-PAGE Saccharopolyspora spinosa
homodimer 2 * 43356, calculated from amino acid sequence Saccharopolyspora spinosa

Synonyms

Synonyms Comment Organism
SpnR
-
 Saccharopolyspora spinosa
TDP-4-keto-2,3,6-trideoxy-D-glucose 4-aminotransferase
-
 Saccharopolyspora spinosa

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Saccharopolyspora spinosa