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Literature summary for 2.6.1.13 extracted from

  • Strecker, H.J.
    Purification and properties of rat liver ornithine DELTA-transaminase (1965), J. Biol. Chem., 240, 1225-1230.
    View publication on PubMed
Search for more literature for 2.6.1.13

General Stability

General Stability Organism
activity of the purified enzyme is preserved by addition of pyridoxal 5'-phosphate Rattus norvegicus
pyridoxal 5'-phosphate stabilizes Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
2-oxobutanoate
-
 Rattus norvegicus
2-oxoglutarate alpha-keto acid acceptor, inhibitor at high concentrations Rattus norvegicus
2-Oxohexanoate
-
 Rattus norvegicus
2-oxoisopentanoate
-
 Rattus norvegicus
4-aminobutanoate
-
 Rattus norvegicus
5-Aminopentanoate
-
 Rattus norvegicus
cadaverine
-
 Rattus norvegicus
DL-norvaline
-
 Rattus norvegicus
glyoxylate alpha-keto acid acceptor, inhibitor at high concentrations Rattus norvegicus
L-canavanine complete inhibition at 0.025 M Rattus norvegicus
L-leucine
-
 Rattus norvegicus
L-valine
-
 Rattus norvegicus
ornithine at high concentrations Rattus norvegicus
putrescine
-
 Rattus norvegicus
pyruvate alpha-keto acid acceptor, inhibitor at high concentrations Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.28
-
 2-oxoglutarate pH 7.1, 37°C Rattus norvegicus
1.7
-
 ornithine with pyruvate as cosubstrate, pH 7.1, 37°C Rattus norvegicus
2.8
-
 ornithine with 2-oxoglutarate as cosubstrate, pH 7.1, 37°C Rattus norvegicus
2.9
-
 ornithine with glyoxylate as cosubstrate, pH 7.1, 37°C Rattus norvegicus
6.7
-
 glyoxylate pH 7.1, 37°C Rattus norvegicus
22
-
 pyruvate pH 7.1, 37°C Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-ornithine + 2-oxoglutarate Rattus norvegicus metabolism of proline to ornithine DELTA1-pyrroline-5-carboxylate + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.21
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
-15°C, rat liver homogenate in sucrose or phosphate solution, stable for some weeks Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-ornithine + 2-oxoglutarate D-ornithine is not accepted as substrate Rattus norvegicus DELTA1-pyrroline-5-carboxylate + L-glutamate equilibrium constant: 71 ?
L-ornithine + 2-oxoglutarate metabolism of proline to ornithine Rattus norvegicus DELTA1-pyrroline-5-carboxylate + L-glutamate
-
 ?
L-ornithine + glyoxylate
-
 Rattus norvegicus DELTA1-pyrroline-5-carboxylate + glycine
-
 ?
L-ornithine + pyruvate
-
 Rattus norvegicus DELTA1-pyrroline-5-carboxylate + L-alanine
-
 ?
additional information inactive as amino group acceptor: 2-oxoisohexanoate Rattus norvegicus ?
-
 ?
additional information inactive as amino group acceptor: 2-oxoisopentanoate Rattus norvegicus ?
-
 ?
additional information inactive as amino group acceptor: oxaloacetate Rattus norvegicus ?
-
 ?
additional information specific for ornithine Rattus norvegicus ?
-
 ?
additional information inactive as amino group acceptor: 2-oxobutanoate Rattus norvegicus ?
-
 ?
additional information inactive as amino group acceptor: 2-oxopentanoate Rattus norvegicus ?
-
 ?
additional information inactive as amino group acceptor: 2-oxohexanoate Rattus norvegicus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus