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Literature summary for 2.6.1.19 extracted from
- Purification and characterization of the 4-aminobutyrate--2,ketoglutarate transaminase from mouse brain (1973), Biochemistry, 12, 2868-2873.
General Stability
| General Stability | Organism |
|---|---|
| freeze-thawing slightly inactivates | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Aminooxyacetate | - |
Mus musculus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | 0.27 | 2-oxoglutarate | 37°C, pH 8.0 | Mus musculus | |
| 1.1 | - |
4-aminobutanoate | 37°C, pH 8.0 | Mus musculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Mus musculus | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 109000 | - |
high speed sedimentation equilibrium centrifugation | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-aminobutanoate + 2-oxoglutarate | Mus musculus | involved in gamma-aminobutyrate metabolism | 4-oxobutanoate + L-glutamate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
Swiss albino | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5 | - |
37°C, pH 8.0 | Mus musculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, protected with 2-aminoethylisothiouronium bromide hydrobromide and pyridoxal phosphate, several months | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-aminoisobutanoate + 2-oxoglutarate | transamination at 55% the rate of 4-aminobutanoate | Mus musculus | L-glutamate + 3-oxoisobutanoate | - |
r | |
| 4-aminobutanoate + 2-oxoglutarate | specific for 2-oxoglutarate | Mus musculus | 4-oxobutanoate + L-glutamate | i.e. succinic semialdehyde + L-Glu | r | |
| 4-aminobutanoate + 2-oxoglutarate | involved in gamma-aminobutyrate metabolism | Mus musculus | 4-oxobutanoate + L-glutamate | - |
? | |
| 5-aminopentanoate + 2-oxoglutarate | transamination at 48% the rate of 4-aminobutanoate | Mus musculus | L-glutamate + 5-oxopentanoate | - |
? | |
| beta-alanine + 2-oxoglutarate | effective amino group donor | Mus musculus | malonic semialdehyde + L-glutamate | - |
r | |
| DL-3-hydroxy-4-aminobutanoate + 2-oxoglutarate | - |
Mus musculus | L-glutamate + 3-hydroxy-4-oxobutanoate | - |
r | |
| additional information | overview | Mus musculus | ? | - |
? | |
| additional information | no amino acceptors are oxaloacetate, pyruvate | Mus musculus | ? | - |
? | |
| additional information | no amino acceptors are 2-oxobutyrate, phenylpyruvate, alpha-ketoadipate | Mus musculus | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.1 | - |
- |
Mus musculus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.9 | 8.8 | about half-maximal activity at pH 6.9 and 8.8 | Mus musculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Mus musculus | |
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