Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.6.1.2 extracted from

  • Gubern, G.; Imperial, S.; Busquets, M.; Cortes, A.
    Partial characterization of the alanine aminotransferase isoenzymes from human liver (1990), Biochem. Soc. Trans., 18, 1288-1289.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
2-oxoglutarate pH 7.8, 30°C, mitochondrial enzyme Homo sapiens
0.22
-
2-oxoglutarate pH 7.8, 30°C, cytosolic enzyme Homo sapiens
5.1
-
L-alanine pH 7.8, 30°C, mitochondrial enzyme Homo sapiens
21
-
alanine pH 7.8, 30°C, cytosolic enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-alanine + 2-oxoglutarate Homo sapiens mitochondrial isoenzyme shows higher affinity for L-alanine, mainly functions in the direction of conversion of alanine to pyruvate whereas the cytosolic isoenzyme would function in both directions pyruvate + L-glutamate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE-Sepharose, partial purification of cytosolic and mitochondrial isoenzymes Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine + 2-oxoglutarate
-
Homo sapiens pyruvate + L-glutamate
-
?
L-alanine + 2-oxoglutarate mitochondrial isoenzyme shows higher affinity for L-alanine, mainly functions in the direction of conversion of alanine to pyruvate whereas the cytosolic isoenzyme would function in both directions Homo sapiens pyruvate + L-glutamate
-
?