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Literature summary for 2.6.1.2 extracted from
- Purification and properties of L-alanine aminotransferase from Chlamydomonas reinhardtii (1991), Eur. J. Biochem., 202, 881-887.
General Stability
| General Stability | Organism |
|---|---|
| pyridoxal 5'-phosphate, L-alanine or 2-oxoglutarate protects against heat inactivation | Chlamydomonas reinhardtii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 2 mM, 51% inhibition | Chlamydomonas reinhardtii |  |
| Aminooxyacetate | 1 mM, complete inhibition | Chlamydomonas reinhardtii | |
| hydroxylamine | 2 mM, 96% inhibition | Chlamydomonas reinhardtii | |
| L-ascorbic acid | 2 mM, 25% inhibition | Chlamydomonas reinhardtii | |
| p-hydroxymercuribenzoate | 1 mM, complete inhibition | Chlamydomonas reinhardtii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
2-oxoglutarate | pH 7.3, 40°C | Chlamydomonas reinhardtii | |
| 0.24 | - |
pyruvate | pH 7.3, 40°C | Chlamydomonas reinhardtii | |
| 0.52 | - |
L-glutamate | pH 7.3, 40°C | Chlamydomonas reinhardtii | |
| 2.7 | - |
L-alanine | pH 7.3, 40°C | Chlamydomonas reinhardtii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
2 * 45000, SDS-PAGE | Chlamydomonas reinhardtii |
| 105000 | - |
gel filtration | Chlamydomonas reinhardtii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlamydomonas reinhardtii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Chlamydomonas reinhardtii |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| L-alanine + 2-oxoglutarate = pyruvate + L-glutamate | ping pong bi bi mechanism | Chlamydomonas reinhardtii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 18.66 | - |
- |
Chlamydomonas reinhardtii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glyoxylate + alanine | - |
Chlamydomonas reinhardtii | glycine + pyruvate | - |
? | |
| glyoxylate + glutamate | - |
Chlamydomonas reinhardtii | glycine + 2-oxoglutarate | - |
? | |
| L-alanine + 2-oxoglutarate | - |
Chlamydomonas reinhardtii | pyruvate + L-glutamate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 45000, SDS-PAGE | Chlamydomonas reinhardtii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Chlamydomonas reinhardtii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
pyridoxal 5-phosphate, L-alanine or 2-oxoglutarate protect against heat inactivation | Chlamydomonas reinhardtii |
| 50 | - |
50% loss of activity after 10 min | Chlamydomonas reinhardtii |
| 60 | - |
more than 85% loss of activity after 5 min | Chlamydomonas reinhardtii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
- |
Chlamydomonas reinhardtii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | a pyridoxal phosphate protein, 2 mol pyridoxal phosphate per mol of enzyme | Chlamydomonas reinhardtii | |
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Release 2023.1 (January 2023)
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