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Literature summary for 2.6.1.21 extracted from
- The partial purification and some properties of D-alanine aminotransferase from Rhizobium japonicum (1973), Biochem. Soc. Trans., 1, 252-254.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-chloromercuribenzoate | 4 mM, 36% inhibition, D-alanine and pyridoxal 5'-phosphate protect to some extent | Bradyrhizobium japonicum |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
2-oxoglutarate | pH 7.9, 30°C | Bradyrhizobium japonicum | |
| 2.4 | - |
D-alanine | pH 7.9, 30°C | Bradyrhizobium japonicum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
gel filtration | Bradyrhizobium japonicum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-alanine + 2-oxoglutarate | Bradyrhizobium japonicum | function of the enzyme is probably the provision of D-amino acids for cell-wall synthesis | pyruvate + D-glutamate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bradyrhizobium japonicum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-cellulose, Sephadex G-200, partial purification | Bradyrhizobium japonicum |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| D-alanine + 2-oxoglutarate = pyruvate + D-glutamate | ping pong mechanism | Bradyrhizobium japonicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-alanine + 2-oxoglutarate | - |
Bradyrhizobium japonicum | pyruvate + D-glutamate | - |
r | |
| D-alanine + 2-oxoglutarate | function of the enzyme is probably the provision of D-amino acids for cell-wall synthesis | Bradyrhizobium japonicum | pyruvate + D-glutamate | - |
? | |
| D-glutamate + 2-oxoglutarate | - |
Bradyrhizobium japonicum | 2-oxoglutarate + D-glutamate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Bradyrhizobium japonicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | 7.9 | - |
Bradyrhizobium japonicum |
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Release 2023.1 (January 2023)
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