Application | Comment | Organism |
---|---|---|
synthesis | biocatalytic transamination with near-stoichiometric inexpensive amine donors mediated by bifunctional mono- and di-amine transaminases. Both monoamine and diamine transaminase activity is reported, allowing conversion of a wide range of target ketone substrates with just a small excess of amine donor. The diamine co-substrates (putrescine, cadaverine or spermidine) are bio-derived and the enzyme system results in very little waste, making it a greener strategy for the production of valuable amine fine chemicals and pharmaceuticals | Pseudomonas aeruginosa |
synthesis | biocatalytic transamination with near-stoichiometric inexpensive amine donors mediated by bifunctional mono- and di-amine transaminases. Both monoamine and diamine transaminase activity is reported, allowing conversion of a wide range of target ketone substrates with just a small excess of amine donor. The diamine co-substrates (putrescine, cadaverine or spermidine) are bio-derived and the enzyme system results in very little waste, making it a greener strategy for the production of valuable amine fine chemicals and pharmaceuticals | Pseudomonas putida |
synthesis | biocatalytic transamination with near-stoichiometric inexpensive amine donors mediated by bifunctional mono- and di-amine transaminases. Both monoamine and diamine transaminase activity is reported, allowing conversion of a wide range of target ketone substrates with just a small excess of amine donor. The diamine co-substrates (putrescine, cadaverine or spermidine) are bio-derived and the enzyme system results in very little waste, making it a greener strategy for the production of valuable amine fine chemicals and pharmaceuticals | Pseudomonas chlororaphis subsp. aureofaciens |
synthesis | biocatalytic transamination with near-stoichiometric inexpensive amine donors mediated by bifunctional mono- and di-amine transaminases. Both monoamine and diamine transaminase activity is reported, allowing conversion of a wide range of target ketone substrates with just a small excess of amine donor. The diamine co-substrates (putrescine, cadaverine or spermidine) are bio-derived and the enzyme system results in very little waste, making it a greener strategy for the production of valuable amine fine chemicals and pharmaceuticals | Pseudomonas fluorescens |
Cloned (Comment) | Organism |
---|---|
gene spuC, recombinant overexpression in Escherichia coli strain BL21(DE3) | Pseudomonas aeruginosa |
gene spuC, recombinant overexpression in Escherichia coli strain BL21(DE3) | Pseudomonas putida |
gene spuC, recombinant overexpression in Escherichia coli strain BL21(DE3) | Pseudomonas chlororaphis subsp. aureofaciens |
gene spuC, recombinant overexpression in Escherichia coli strain BL21(DE3) | Pseudomonas fluorescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
an alpha,omega-diamine + 2-oxoglutarate | Pseudomonas aeruginosa | - |
an omega-aminoaldehyde + L-glutamate | - |
? | |
an alpha,omega-diamine + 2-oxoglutarate | Pseudomonas putida | - |
an omega-aminoaldehyde + L-glutamate | - |
? | |
an alpha,omega-diamine + 2-oxoglutarate | Pseudomonas chlororaphis subsp. aureofaciens | - |
an omega-aminoaldehyde + L-glutamate | - |
? | |
an alpha,omega-diamine + 2-oxoglutarate | Pseudomonas fluorescens | - |
an omega-aminoaldehyde + L-glutamate | - |
? | |
an alpha,omega-diamine + 2-oxoglutarate | Pseudomonas aeruginosa ATCC 15692 | - |
an omega-aminoaldehyde + L-glutamate | - |
? | |
an alpha,omega-diamine + 2-oxoglutarate | Pseudomonas fluorescens F113 | - |
an omega-aminoaldehyde + L-glutamate | - |
? | |
an alpha,omega-diamine + 2-oxoglutarate | Pseudomonas aeruginosa 1C | - |
an omega-aminoaldehyde + L-glutamate | - |
? | |
an alpha,omega-diamine + 2-oxoglutarate | Pseudomonas aeruginosa PRS 101 | - |
an omega-aminoaldehyde + L-glutamate | - |
? | |
an alpha,omega-diamine + 2-oxoglutarate | Pseudomonas aeruginosa DSM 22644 | - |
an omega-aminoaldehyde + L-glutamate | - |
? | |
an alpha,omega-diamine + 2-oxoglutarate | Pseudomonas aeruginosa CIP 104116 | - |
an omega-aminoaldehyde + L-glutamate | - |
? | |
an alpha,omega-diamine + 2-oxoglutarate | Pseudomonas aeruginosa LMG 12228 | - |
an omega-aminoaldehyde + L-glutamate | - |
? | |
an alpha,omega-diamine + 2-oxoglutarate | Pseudomonas aeruginosa JCM 14847 | - |
an omega-aminoaldehyde + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9I6J2 | - |
- |
Pseudomonas aeruginosa 1C | Q9I6J2 | - |
- |
Pseudomonas aeruginosa ATCC 15692 | Q9I6J2 | - |
- |
Pseudomonas aeruginosa CIP 104116 | Q9I6J2 | - |
- |
Pseudomonas aeruginosa DSM 22644 | Q9I6J2 | - |
- |
Pseudomonas aeruginosa JCM 14847 | Q9I6J2 | - |
- |
Pseudomonas aeruginosa LMG 12228 | Q9I6J2 | - |
- |
Pseudomonas aeruginosa PRS 101 | Q9I6J2 | - |
- |
Pseudomonas chlororaphis subsp. aureofaciens | A0A290U4C9 | - |
- |
Pseudomonas fluorescens | G8Q4R3 | - |
- |
Pseudomonas fluorescens F113 | G8Q4R3 | - |
- |
Pseudomonas putida | A0A4P8GNL5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
an alpha,omega-diamine + 2-oxoglutarate | - |
Pseudomonas aeruginosa | an omega-aminoaldehyde + L-glutamate | - |
? | |
an alpha,omega-diamine + 2-oxoglutarate | - |
Pseudomonas putida | an omega-aminoaldehyde + L-glutamate | - |
? | |
an alpha,omega-diamine + 2-oxoglutarate | - |
Pseudomonas chlororaphis subsp. aureofaciens | an omega-aminoaldehyde + L-glutamate | - |
? | |
an alpha,omega-diamine + 2-oxoglutarate | - |
Pseudomonas fluorescens | an omega-aminoaldehyde + L-glutamate | - |
? | |
an alpha,omega-diamine + 2-oxoglutarate | - |
Pseudomonas aeruginosa ATCC 15692 | an omega-aminoaldehyde + L-glutamate | - |
? | |
an alpha,omega-diamine + 2-oxoglutarate | - |
Pseudomonas fluorescens F113 | an omega-aminoaldehyde + L-glutamate | - |
? | |
an alpha,omega-diamine + 2-oxoglutarate | - |
Pseudomonas aeruginosa 1C | an omega-aminoaldehyde + L-glutamate | - |
? | |
an alpha,omega-diamine + 2-oxoglutarate | - |
Pseudomonas aeruginosa PRS 101 | an omega-aminoaldehyde + L-glutamate | - |
? | |
an alpha,omega-diamine + 2-oxoglutarate | - |
Pseudomonas aeruginosa DSM 22644 | an omega-aminoaldehyde + L-glutamate | - |
? | |
an alpha,omega-diamine + 2-oxoglutarate | - |
Pseudomonas aeruginosa CIP 104116 | an omega-aminoaldehyde + L-glutamate | - |
? | |
an alpha,omega-diamine + 2-oxoglutarate | - |
Pseudomonas aeruginosa LMG 12228 | an omega-aminoaldehyde + L-glutamate | - |
? | |
an alpha,omega-diamine + 2-oxoglutarate | - |
Pseudomonas aeruginosa JCM 14847 | an omega-aminoaldehyde + L-glutamate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha,omega-diamine transaminase | - |
Pseudomonas aeruginosa |
alpha,omega-diamine transaminase | - |
Pseudomonas putida |
alpha,omega-diamine transaminase | - |
Pseudomonas chlororaphis subsp. aureofaciens |
alpha,omega-diamine transaminase | - |
Pseudomonas fluorescens |
alpha,omega-DTA | - |
Pseudomonas aeruginosa |
alpha,omega-DTA | - |
Pseudomonas putida |
bifunctional di-amine transaminase | - |
Pseudomonas aeruginosa |
bifunctional mono- and di-amine transaminase | - |
Pseudomonas putida |
bifunctional mono- and di-amine transaminase | - |
Pseudomonas chlororaphis subsp. aureofaciens |
bifunctional mono- and di-amine transaminase | - |
Pseudomonas fluorescens |
Pa-SpuC | - |
Pseudomonas aeruginosa |
Pc-SpuC | - |
Pseudomonas chlororaphis subsp. aureofaciens |
Pf-SpuC | - |
Pseudomonas fluorescens |
Pp-SpuC | - |
Pseudomonas putida |
SpuC | - |
Pseudomonas aeruginosa |
SpuC | - |
Pseudomonas putida |
SpuC | - |
Pseudomonas chlororaphis subsp. aureofaciens |
SpuC | - |
Pseudomonas fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Pseudomonas aeruginosa | |
pyridoxal 5'-phosphate | - |
Pseudomonas putida | |
pyridoxal 5'-phosphate | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
pyridoxal 5'-phosphate | - |
Pseudomonas fluorescens |