Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | activation only in the presence of pyridoxal phosphate | Rattus norvegicus | |
2-mercaptoethanol | activation only in the presence of pyridoxal phosphate | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hydroxylamine | - |
Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.81 | - |
beta-Alanine | pH 8.8, 37°C | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-amino-2-methylpropanoate + pyruvate | Cavia porcellus | first step in aminoisobutyrate metabolism | 2-methyl-3-oxopropanoate + L-alanine | - |
? | |
(R)-3-amino-2-methylpropanoate + pyruvate | Homo sapiens | first step in aminoisobutyrate metabolism | 2-methyl-3-oxopropanoate + L-alanine | - |
? | |
(R)-3-amino-2-methylpropanoate + pyruvate | Rattus norvegicus | first step in aminoisobutyrate metabolism | 2-methyl-3-oxopropanoate + L-alanine | - |
? | |
(R)-3-amino-2-methylpropanoate + pyruvate | Sus scrofa | first step in aminoisobutyrate metabolism | 2-methyl-3-oxopropanoate + L-alanine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cavia porcellus | - |
- |
- |
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Cavia porcellus | - |
liver | - |
Cavia porcellus | - |
liver | - |
Homo sapiens | - |
liver | - |
Rattus norvegicus | - |
liver | - |
Sus scrofa | - |
additional information | - |
Rattus norvegicus | - |
additional information | distribution in guinea pig tissue | Cavia porcellus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.093 | - |
pH 8.8, 37°C | Sus scrofa |
Storage Stability | Organism |
---|---|
-20°C, at least 1 month | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-amino-2-methylpropanoate + glyoxylate | - |
Rattus norvegicus | 2-methyl-3-oxopropanoate + glycine | - |
? | |
(R)-3-amino-2-methylpropanoate + glyoxylate | with 50% efficiency compared to pyruvate | Sus scrofa | 2-methyl-3-oxopropanoate + glycine | - |
? | |
(R)-3-amino-2-methylpropanoate + pyruvate | - |
Homo sapiens | 2-methyl-3-oxopropanoate + L-alanine | - |
? | |
(R)-3-amino-2-methylpropanoate + pyruvate | - |
Sus scrofa | 2-methyl-3-oxopropanoate + L-alanine | - |
? | |
(R)-3-amino-2-methylpropanoate + pyruvate | - |
Rattus norvegicus | 2-methyl-3-oxopropanoate + L-alanine | i.e. methylmalonyl semialdehyde | ? | |
(R)-3-amino-2-methylpropanoate + pyruvate | best substrate among omega-amino acids, highly specific for D-isomer | Cavia porcellus | 2-methyl-3-oxopropanoate + L-alanine | - |
r | |
(R)-3-amino-2-methylpropanoate + pyruvate | first step in aminoisobutyrate metabolism | Cavia porcellus | 2-methyl-3-oxopropanoate + L-alanine | - |
? | |
(R)-3-amino-2-methylpropanoate + pyruvate | first step in aminoisobutyrate metabolism | Homo sapiens | 2-methyl-3-oxopropanoate + L-alanine | - |
? | |
(R)-3-amino-2-methylpropanoate + pyruvate | first step in aminoisobutyrate metabolism | Rattus norvegicus | 2-methyl-3-oxopropanoate + L-alanine | - |
? | |
(R)-3-amino-2-methylpropanoate + pyruvate | first step in aminoisobutyrate metabolism | Sus scrofa | 2-methyl-3-oxopropanoate + L-alanine | - |
? | |
4-aminobutanoate + pyruvate | transamination at 19% the rate of (R)-3-amino-2-methylpropanoate | Sus scrofa | succinic semialdehyde + L-alanine | - |
? | |
beta-alanine + pyruvate | - |
Rattus norvegicus | malonic semialdehyde + L-alanine | - |
? | |
beta-alanine + pyruvate | transamination at 66% the rate of (R)-3-amino-2-methylpropanoate | Sus scrofa | malonic semialdehyde + L-alanine | - |
? | |
DL-alpha-ethyl-beta-alanine + pyruvate | transamination at 33% the rate of (R)-3-amino-2-methylpropanoate | Sus scrofa | 2-ethyl-3-oxopropanoate + L-alanine | - |
? | |
additional information | - |
Cavia porcellus | ? | - |
? | |
additional information | - |
Homo sapiens | ? | - |
? | |
additional information | no substrate: 2-oxoglutarate | Rattus norvegicus | ? | - |
? | |
additional information | no substrate: 2-oxoglutarate | Sus scrofa | ? | - |
? | |
additional information | no substrate: 5-amino-n-pentanoate, 6-aminohexanoate, DL-3-aminobutyrate, oxaloacetate | Sus scrofa | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Cavia porcellus |
37 | - |
assay at | Homo sapiens |
37 | - |
assay at | Rattus norvegicus |
37 | - |
assay at | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Sus scrofa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 10.6 | about half-maximal activity at pH 7.5 and pH 10.6 | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Rattus norvegicus | |
pyridoxal 5'-phosphate | - |
Sus scrofa |