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Literature summary for 2.6.1.40 extracted from

  • Ueno, S.I.; Morino, H.; Sano, A.; Kakimoto, Y.
    Purification and characterization of D-3-aminoisobutyrate-pyruvate aminotransferase from rat liver (1990), Biochim. Biophys. Acta, 1033, 169-175.
    View publication on PubMed
Search for more literature for 2.6.1.40

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol activation only in the presence of pyridoxal phosphate Rattus norvegicus
DTT activation Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
GSH weak Rattus norvegicus
L-cysteine
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Rattus norvegicus 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 4 * 50000, reductive SDS-PAGE Rattus norvegicus
180000
-
 gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14
-
 pH 8.8, 37°C Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, concentrated enzyme solution, in 5% polyethylene glycol plus 1 mg/ml pyridoxal 5'-phosphate, several months Rattus norvegicus
4°C, concentrated enzyme solution, several days Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-3-amino-2-methylpropanoate + 2-oxo-n-butanoate
-
 Rattus norvegicus 2-methyl-3-oxopropanoate + 2-amino-n-butanoate
-
 ?
(R)-3-amino-2-methylpropanoate + 2-oxo-n-pentanoate
-
 Rattus norvegicus 2-methyl-3-oxopropanoate + norvaline
-
 ?
(R)-3-amino-2-methylpropanoate + glyoxylate
-
 Rattus norvegicus 2-methyl-3-oxopropanoate + glycine
-
 ?
(R)-3-amino-2-methylpropanoate + oxaloacetate
-
 Rattus norvegicus 2-methyl-3-oxopropanoate + L-aspartate
-
 ?
(R)-3-amino-2-methylpropanoate + pyruvate
-
 Rattus norvegicus 2-methyl-3-oxopropanoate + L-alanine
-
 ?
5-aminolevulinic acid + pyruvate
-
 Rattus norvegicus 5-oxolevulinic acid + L-alanine
-
 ?
arginine + pyruvate transamination at 40% the rate of (R)-3-amino-2-methylpropanoate Rattus norvegicus 2-oxo-5-guanidinovaleric acid + L-alanine
-
 ?
beta-alanine + pyruvate
-
 Rattus norvegicus malonic semialdehyde + L-alanine
-
 ?
DL-3-amino-n-butanoate + pyruvate
-
 Rattus norvegicus 3-oxobutanoate + L-alanine
-
 ?
glycine + pyruvate
-
 Rattus norvegicus glyoxylate + L-alanine
-
 ?
additional information overview Rattus norvegicus ?
-
 ?
NG,NG'-dimethyl-(S)-arginine + pyruvate
-
 Rattus norvegicus ? + L-alanine
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 50000, reductive SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate requirement, tightly bound Rattus norvegicus