General Stability | Organism |
---|---|
pyridoxal 5'-phosphate stabilizes | Arachis hypogaea |
reducing agents, such as cysteine, 2-mercaptoethanol or dithiothreitol, are essential for maintaining activity during purification | Arachis hypogaea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arachis hypogaea | - |
peanut cv. Starr | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, cold precipitation, acetone precipitation, partial purification | Arachis hypogaea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | germinating | Arachis hypogaea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
10061 units/mg, 1 unit is defined as the quantity of enzyme that produces 1 nanoliter of ethylene per h of dark reaction | Arachis hypogaea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-methionine + 2-oxoglutarate | less effective than pyruvate | Arachis hypogaea | 4-methylthio-2-oxobutanoate + L-glutamate | - |
? | |
D-methionine + oxaloacetate | less effective than pyruvate | Arachis hypogaea | 4-methylthio-2-oxobutanoate + L-aspartate | - |
? | |
D-methionine + pyruvate | - |
Arachis hypogaea | 4-methylthio-2-oxobutanoate + L-alanine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
31 | - |
assay at | Arachis hypogaea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Arachis hypogaea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | required for full enzyme activity | Arachis hypogaea |