Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermococcus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Aminooxyacetate | residual activity 79% | Thermococcus sp. | |
DL-Penicillamine | residual activity 79% | Thermococcus sp. | |
hydroxylamine | residual activity 72% | Thermococcus sp. | |
Semicarbazide | residual activity 95% | Thermococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.034 | - |
2-oxo-3-methylvalerate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
0.06 | - |
2-oxoisocaproate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
0.06 | - |
2-oxocaproate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
0.15 | - |
L-isoleucine | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
0.16 | - |
2-oxovalerate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
0.18 | - |
2-oxoisovalerate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
0.34 | - |
phenylpyruvate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
0.55 | - |
2-oxobutanoate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
0.73 | - |
DL-2-aminooctanoate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
0.83 | - |
L-valine | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
1.2 | - |
L-leucine | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
1.7 | - |
2-oxoglutarate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
1.9 | - |
L-norvaline | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2 | - |
DL-2-aminoheptanoate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.4 | - |
L-2-aminobutanoate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
3.9 | - |
L-glutamate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
4.2 | - |
L-phenylalanine | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
12 | - |
L-norleucine | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus sp. | A0A060PQX5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-3-methylvalerate + glyoxalate | - |
Thermococcus sp. | L-isoleucine + glycine | - |
r | |
DL-2-aminoheptanoate + 2-oxoglutarate | - |
Thermococcus sp. | 2-oxoheptanoate + L-glutamate | - |
r | |
DL-2-aminooctanoate + 2-oxobutanoate | - |
Thermococcus sp. | 2-oxooctanoate + L-2-aminobutanoate | - |
r | |
L-2-aminobutanoate + phenylpyruvate | - |
Thermococcus sp. | 2-oxobutanoate + L-phenylalanine | - |
r | |
L-glutamate + glyoxalate | - |
Thermococcus sp. | 2-oxoglutarate + glycine | - |
r | |
L-isoleucine + 2-oxoisocaproate | - |
Thermococcus sp. | 3-methyl-2-oxopentanoate + L-leucine | - |
r | |
L-leucine + 2-oxobutanoate | - |
Thermococcus sp. | 2-oxoisohexanoate + L-valine | - |
r | |
L-norleucine + 2-oxoisocaproate | - |
Thermococcus sp. | 2-oxohexanoate + L-leucine | - |
r | |
L-norvaline + 2-oxocaproate | - |
Thermococcus sp. | 2-oxopentanoate + 2-aminohexanoate | - |
r | |
L-phenylalanine + 2-oxovalerate | - |
Thermococcus sp. | phenylpyruvate + L-norvaline | - |
r | |
L-valine + 2-oxoisovalerate | - |
Thermococcus sp. | 2-oxoisovalerate + L-leucine | - |
r |
Synonyms | Comment | Organism |
---|---|---|
BcaT | - |
Thermococcus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
- |
Thermococcus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
stable for at least 5 h | Thermococcus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
25 | - |
L-2-aminobutanoate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
27 | - |
DL-2-aminoheptanoate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
31 | - |
L-valine | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
51 | - |
L-isoleucine | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
52 | - |
phenylpyruvate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
80 | - |
L-phenylalanine | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
81 | - |
DL-2-aminooctanoate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
86 | - |
L-norvaline | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
91 | - |
2-oxocaproate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
110 | - |
L-leucine | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
150 | - |
L-glutamate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
190 | - |
2-oxovalerate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
250 | - |
2-oxobutanoate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
260 | - |
L-norleucine | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
270 | - |
2-oxoisovalerate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
270 | - |
2-oxoisocaproate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
360 | - |
2-oxo-3-methylvalerate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
700 | - |
2-oxoglutarate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Thermococcus sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | - |
Thermococcus sp. |
General Information | Comment | Organism |
---|---|---|
physiological function | Thermococcus sp. CKU-1 requires L-leucine, L-methionine, L-phenylalanine, L-threonine, L-tryptophan, and L-tyrosine for its growth, which are good substrates for BcAT. The enzyme might be involved in the degradation of these amino acids | Thermococcus sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11 | - |
L-2-aminobutanoate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
13 | - |
DL-2-aminoheptanoate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
19 | - |
L-phenylalanine | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
21 | - |
L-norleucine | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
38 | - |
L-valine | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
39 | - |
L-glutamate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
45 | - |
L-norvaline | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
87 | - |
L-leucine | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
110 | - |
DL-2-aminooctanoate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
150 | - |
phenylpyruvate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
350 | - |
L-isoleucine | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
430 | - |
2-oxoglutarate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
460 | - |
2-oxobutanoate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
1200 | - |
2-oxovalerate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
1500 | - |
2-oxoisovalerate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
1500 | - |
2-oxocaproate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
4600 | - |
2-oxoisocaproate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
11000 | - |
2-oxo-3-methylvalerate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. |