KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.08 | - |
3-methyl-2-oxopentanoate | pH 8.3, 337°C | Arabidopsis thaliana | |
2.64 | - |
4-methyl-2-oxopentanoate | pH 8.3, 337°C | Arabidopsis thaliana | |
2.69 | - |
3-methyl-2-oxobutanoate | pH 8.3, 337°C | Arabidopsis thaliana | |
2.83 | - |
4-methylthio-2-oxobutanoate | pH 8.3, 337°C | Arabidopsis thaliana | |
3.27 | - |
L-isoleucine | pH 8.3, 337°C | Arabidopsis thaliana | |
3.34 | - |
L-leucine | pH 8.3, 337°C | Arabidopsis thaliana | |
4.41 | - |
L-valine | pH 8.3, 337°C | Arabidopsis thaliana | |
6.58 | - |
L-methionine | pH 8.3, 337°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Arabidopsis thaliana | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9LPM9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + L-isoleucine | - |
Arabidopsis thaliana | L-glutamate + 3-methyl-2-oxopentanoate | - |
r | |
2-oxoglutarate + L-leucine | - |
Arabidopsis thaliana | L-glutamate + 4-methyl-2-oxopentanoate | - |
r | |
3-methyl-2-oxobutanoate + L-glutamate | - |
Arabidopsis thaliana | L-valine + 2-oxoglutarate | - |
r | |
4-methylthio-2-oxobutanoate + L-glutamate | - |
Arabidopsis thaliana | L-methionine + 2-oxoglutarate | - |
r | |
L-glutamate + 3-methyl-2-oxopentanoate | - |
Arabidopsis thaliana | 2-oxoglutarate + L-isoleucine | - |
r | |
L-glutamate + 4-methyl-2-oxopentanoate | - |
Arabidopsis thaliana | 2-oxoglutarate + L-leucine | - |
r | |
L-methionine + 2-oxoglutarate | - |
Arabidopsis thaliana | 4-methylthio-2-oxobutanoate + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | - |
Arabidopsis thaliana | 3-methyl-2-oxobutanoate + L-glutamate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
At1g50110 | locus name | Arabidopsis thaliana |
Bcat6 | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
physiological function | mutant plants lacking the activities of isoforms bcat3, Bcat4, Bcat6 exhibit a clear macroscopic phenotype with smaller plants and abnormal leaf morphology. The triple mutant shows a dramatic reduction of Met-derived glucosinolate species down to 32 and 14% of wild-type levels in plant foliage and seeds, respectively, accompanied by a 46fold increase of free Met. 5?-Deoxy-5?-methylthioadenosine, an intermediate of the Met recycling pathway, accumulates to relative high amounts in the absence of the cytosolic Bcat4 and Bcat6 | Arabidopsis thaliana |