Literature summary for 2.6.1.42 extracted from

Bezsudnova, E.Y.; Dibrova, D.V.; Nikolaeva, A.Y.; Rakitina, T.V.; Popov, V.O.
Identification of branched-chain amino acid aminotransferases active towards (R)-(+)-1-phenylethylamine among PLP fold type IV transaminases (2018), J. Biotechnol., 271, 26-28 .

Cloned(Commentary)

Cloned (Comment)	Organism
gene ilvE, recombinant expression of codon-optimized gene encoding the His6-tagged enzyme with TEV proteae cleavage site in Escherichia coli strain BL21(DE3)pLys	Methanococcus vannielii
gene ilvE, recombinant expression of codon-optimized gene encoding the His6-tagged enzyme with TEV proteae cleavage site in Escherichia coli strain BL21(DE3)pLys	Thermomicrobium roseum
gene ilvE, recombinant expression of codon-optimized gene encoding the His6-tagged enzyme with TEV protease cleavage site in Escherichia coli strain BL21(DE3)pLys	Thermobaculum terrenum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
10.3	-	(R)-1-phenylethylamine	pH 9.0, 50°C, recombinant enzyme, with 2-oxogluarate	Thermobaculum terrenum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
L-leucine + 2-oxoglutarate	Methanococcus vannielii	-	4-methyl-2-oxopentanoate + L-glutamate	-	r
L-leucine + 2-oxoglutarate	Thermomicrobium roseum	-	4-methyl-2-oxopentanoate + L-glutamate	-	r
L-leucine + 2-oxoglutarate	Thermobaculum terrenum	-	4-methyl-2-oxopentanoate + L-glutamate	-	r
L-leucine + 2-oxoglutarate	Methanococcus vannielii OCM 148	-	4-methyl-2-oxopentanoate + L-glutamate	-	r
L-leucine + 2-oxoglutarate	Thermomicrobium roseum P-2	-	4-methyl-2-oxopentanoate + L-glutamate	-	r
L-leucine + 2-oxoglutarate	Methanococcus vannielii JCM 13029	-	4-methyl-2-oxopentanoate + L-glutamate	-	r
L-leucine + 2-oxoglutarate	Methanococcus vannielii SB	-	4-methyl-2-oxopentanoate + L-glutamate	-	r
L-leucine + 2-oxoglutarate	Thermomicrobium roseum ATCC 27502	-	4-methyl-2-oxopentanoate + L-glutamate	-	r
L-leucine + 2-oxoglutarate	Methanococcus vannielii ATCC 35089	-	4-methyl-2-oxopentanoate + L-glutamate	-	r
L-leucine + 2-oxoglutarate	Methanococcus vannielii DSM 1224	-	4-methyl-2-oxopentanoate + L-glutamate	-	r
L-leucine + 2-oxoglutarate	Thermomicrobium roseum DSM 5159	-	4-methyl-2-oxopentanoate + L-glutamate	-	r
L-leucine + 2-oxoglutarate	Thermobaculum terrenum YNP1	-	4-methyl-2-oxopentanoate + L-glutamate	-	r

Organism

Organism	UniProt	Comment	Textmining
Methanococcus vannielii	A6UP94	-	-
Methanococcus vannielii ATCC 35089	A6UP94	-	-
Methanococcus vannielii DSM 1224	A6UP94	-	-
Methanococcus vannielii JCM 13029	A6UP94	-	-
Methanococcus vannielii OCM 148	A6UP94	-	-
Methanococcus vannielii SB	A6UP94	-	-
Thermobaculum terrenum	D1CCW1	-	-
Thermobaculum terrenum YNP1	D1CCW1	-	-
Thermomicrobium roseum	B9L0B7	-	-
Thermomicrobium roseum ATCC 27502	B9L0B7	-	-
Thermomicrobium roseum DSM 5159	B9L0B7	-	-
Thermomicrobium roseum P-2	B9L0B7	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)pLys by nickel affinity chromatography, tag cleavage by TEV protease, and gel filtration, to homogeneity	Methanococcus vannielii
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)pLys by nickel affinity chromatography, tag cleavage by TEV protease, and gel filtration, to homogeneity	Thermomicrobium roseum
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)pLys by nickel affinity chromatography, tag cleavage by TEV protease, and gel filtration, to homogeneity	Thermobaculum terrenum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.007	-	purified recombinant enzyme, pH 8.0, 50°C, substrates (R)-1-phenylethylamine and pyruvate	Methanococcus vannielii
0.012	-	purified recombinant enzyme, pH 8.0, 50°C, substrates (R)-1-phenylethylamine and pyruvate	Thermobaculum terrenum
0.012	-	purified recombinant enzyme, pH 9.0, 50°C, substrates (R)-1-phenylethylamine and 2-oxoglutarate	Thermobaculum terrenum
0.033	-	purified recombinant enzyme, pH 8.0, 50°C, substrates (R)-1-phenylethylamine and pyruvate	Thermomicrobium roseum
0.035	-	purified recombinant enzyme, pH 9.0, 50°C, substrates (R)-1-phenylethylamine and 2-oxoglutarate	Methanococcus vannielii
0.15	-	purified recombinant enzyme, pH 9.0, 50°C, substrates (R)-1-phenylethylamine and 2-oxoglutarate	Thermomicrobium roseum
3	-	purified recombinant enzyme, pH 8.0, 50°C, substrates L-leucine and 2-oxoglutarate	Methanococcus vannielii
37	-	purified recombinant enzyme, pH 8.0, 40°C, substrates L-leucine and 2-oxoglutarate	Thermomicrobium roseum
42	-	purified recombinant enzyme, pH 8.0, 40°C, substrates L-leucine and 2-oxoglutarate	Thermobaculum terrenum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
(R)-1-phenylethylamine + 2-oxoglutarate	-	Methanococcus vannielii	acetophenone + L-glutamate	-	r
(R)-1-phenylethylamine + 2-oxoglutarate	-	Thermomicrobium roseum	acetophenone + L-glutamate	-	r
(R)-1-phenylethylamine + 2-oxoglutarate	-	Thermobaculum terrenum	acetophenone + L-glutamate	-	r
(R)-1-phenylethylamine + 2-oxoglutarate	-	Methanococcus vannielii OCM 148	acetophenone + L-glutamate	-	r
(R)-1-phenylethylamine + 2-oxoglutarate	-	Thermomicrobium roseum P-2	acetophenone + L-glutamate	-	r
(R)-1-phenylethylamine + 2-oxoglutarate	-	Methanococcus vannielii JCM 13029	acetophenone + L-glutamate	-	r
(R)-1-phenylethylamine + 2-oxoglutarate	-	Methanococcus vannielii SB	acetophenone + L-glutamate	-	r
(R)-1-phenylethylamine + 2-oxoglutarate	-	Thermomicrobium roseum ATCC 27502	acetophenone + L-glutamate	-	r
(R)-1-phenylethylamine + 2-oxoglutarate	-	Methanococcus vannielii ATCC 35089	acetophenone + L-glutamate	-	r
(R)-1-phenylethylamine + 2-oxoglutarate	-	Methanococcus vannielii DSM 1224	acetophenone + L-glutamate	-	r
(R)-1-phenylethylamine + 2-oxoglutarate	-	Thermomicrobium roseum DSM 5159	acetophenone + L-glutamate	-	r
(R)-1-phenylethylamine + 2-oxoglutarate	-	Thermobaculum terrenum YNP1	acetophenone + L-glutamate	-	r
(R)-1-phenylethylamine + pyruvate	(R)-PEA	Methanococcus vannielii	acetophenone + L-alanine	-	r
(R)-1-phenylethylamine + pyruvate	(R)-PEA	Thermomicrobium roseum	acetophenone + L-alanine	-	r
(R)-1-phenylethylamine + pyruvate	(R)-PEA	Thermobaculum terrenum	acetophenone + L-alanine	-	r
(R)-1-phenylethylamine + pyruvate	(R)-PEA	Methanococcus vannielii OCM 148	acetophenone + L-alanine	-	r
(R)-1-phenylethylamine + pyruvate	(R)-PEA	Thermomicrobium roseum P-2	acetophenone + L-alanine	-	r
(R)-1-phenylethylamine + pyruvate	(R)-PEA	Methanococcus vannielii JCM 13029	acetophenone + L-alanine	-	r
(R)-1-phenylethylamine + pyruvate	(R)-PEA	Methanococcus vannielii SB	acetophenone + L-alanine	-	r
(R)-1-phenylethylamine + pyruvate	(R)-PEA	Thermomicrobium roseum ATCC 27502	acetophenone + L-alanine	-	r
(R)-1-phenylethylamine + pyruvate	(R)-PEA	Methanococcus vannielii ATCC 35089	acetophenone + L-alanine	-	r
(R)-1-phenylethylamine + pyruvate	(R)-PEA	Methanococcus vannielii DSM 1224	acetophenone + L-alanine	-	r
(R)-1-phenylethylamine + pyruvate	(R)-PEA	Thermomicrobium roseum DSM 5159	acetophenone + L-alanine	-	r
(R)-1-phenylethylamine + pyruvate	(R)-PEA	Thermobaculum terrenum YNP1	acetophenone + L-alanine	-	r
L-leucine + 2-oxoglutarate	-	Methanococcus vannielii	4-methyl-2-oxopentanoate + L-glutamate	-	r
L-leucine + 2-oxoglutarate	-	Thermomicrobium roseum	4-methyl-2-oxopentanoate + L-glutamate	-	r
L-leucine + 2-oxoglutarate	-	Thermobaculum terrenum	4-methyl-2-oxopentanoate + L-glutamate	-	r
L-leucine + 2-oxoglutarate	-	Methanococcus vannielii OCM 148	4-methyl-2-oxopentanoate + L-glutamate	-	r
L-leucine + 2-oxoglutarate	-	Thermomicrobium roseum P-2	4-methyl-2-oxopentanoate + L-glutamate	-	r
L-leucine + 2-oxoglutarate	-	Methanococcus vannielii JCM 13029	4-methyl-2-oxopentanoate + L-glutamate	-	r
L-leucine + 2-oxoglutarate	-	Methanococcus vannielii SB	4-methyl-2-oxopentanoate + L-glutamate	-	r
L-leucine + 2-oxoglutarate	-	Thermomicrobium roseum ATCC 27502	4-methyl-2-oxopentanoate + L-glutamate	-	r
L-leucine + 2-oxoglutarate	-	Methanococcus vannielii ATCC 35089	4-methyl-2-oxopentanoate + L-glutamate	-	r
L-leucine + 2-oxoglutarate	-	Methanococcus vannielii DSM 1224	4-methyl-2-oxopentanoate + L-glutamate	-	r
L-leucine + 2-oxoglutarate	-	Thermomicrobium roseum DSM 5159	4-methyl-2-oxopentanoate + L-glutamate	-	r
L-leucine + 2-oxoglutarate	-	Thermobaculum terrenum YNP1	4-methyl-2-oxopentanoate + L-glutamate	-	r
additional information	substrate specificity, overview. The enzyme is active with (S)-amine substrates, but also with (R)-amines to a lower extent. No activity with beta-Ala and D-Ala. The enzyme is active towards (R)-PEA and not active towards (S)-PEA. Reaction mechanisms	Methanococcus vannielii	?	-	-
additional information	substrate specificity, overview. The enzyme is active with (S)-amine substrates, but also with (R)-amines to a lower extent. No activity with beta-Ala and D-Ala. The enzyme is active towards (R)-PEA and not active towards (S)-PEA. Reaction mechanisms	Thermomicrobium roseum	?	-	-
additional information	substrate specificity, overview. The enzyme is active with (S)-amine substrates, but also with (R)-amines to a lower extent. No activity with beta-Ala and D-Ala. The enzyme is active towards (R)-PEA and not active towards (S)-PEA. Reaction mechanisms	Thermobaculum terrenum	?	-	-
additional information	substrate specificity, overview. The enzyme is active with (S)-amine substrates, but also with (R)-amines to a lower extent. No activity with beta-Ala and D-Ala. The enzyme is active towards (R)-PEA and not active towards (S)-PEA. Reaction mechanisms	Methanococcus vannielii OCM 148	?	-	-
additional information	substrate specificity, overview. The enzyme is active with (S)-amine substrates, but also with (R)-amines to a lower extent. No activity with beta-Ala and D-Ala. The enzyme is active towards (R)-PEA and not active towards (S)-PEA. Reaction mechanisms	Thermomicrobium roseum P-2	?	-	-
additional information	substrate specificity, overview. The enzyme is active with (S)-amine substrates, but also with (R)-amines to a lower extent. No activity with beta-Ala and D-Ala. The enzyme is active towards (R)-PEA and not active towards (S)-PEA. Reaction mechanisms	Methanococcus vannielii JCM 13029	?	-	-
additional information	substrate specificity, overview. The enzyme is active with (S)-amine substrates, but also with (R)-amines to a lower extent. No activity with beta-Ala and D-Ala. The enzyme is active towards (R)-PEA and not active towards (S)-PEA. Reaction mechanisms	Methanococcus vannielii SB	?	-	-
additional information	substrate specificity, overview. The enzyme is active with (S)-amine substrates, but also with (R)-amines to a lower extent. No activity with beta-Ala and D-Ala. The enzyme is active towards (R)-PEA and not active towards (S)-PEA. Reaction mechanisms	Thermomicrobium roseum ATCC 27502	?	-	-
additional information	substrate specificity, overview. The enzyme is active with (S)-amine substrates, but also with (R)-amines to a lower extent. No activity with beta-Ala and D-Ala. The enzyme is active towards (R)-PEA and not active towards (S)-PEA. Reaction mechanisms	Methanococcus vannielii ATCC 35089	?	-	-
additional information	substrate specificity, overview. The enzyme is active with (S)-amine substrates, but also with (R)-amines to a lower extent. No activity with beta-Ala and D-Ala. The enzyme is active towards (R)-PEA and not active towards (S)-PEA. Reaction mechanisms	Methanococcus vannielii DSM 1224	?	-	-
additional information	substrate specificity, overview. The enzyme is active with (S)-amine substrates, but also with (R)-amines to a lower extent. No activity with beta-Ala and D-Ala. The enzyme is active towards (R)-PEA and not active towards (S)-PEA. Reaction mechanisms	Thermomicrobium roseum DSM 5159	?	-	-
additional information	substrate specificity, overview. The enzyme is active with (S)-amine substrates, but also with (R)-amines to a lower extent. No activity with beta-Ala and D-Ala. The enzyme is active towards (R)-PEA and not active towards (S)-PEA. Reaction mechanisms	Thermobaculum terrenum YNP1	?	-	-

Synonyms

Synonyms	Comment	Organism
BcaT	-	Methanococcus vannielii
BcaT	-	Thermomicrobium roseum
BcaT	-	Thermobaculum terrenum
branched-chain amino acid aminotransferase	-	Methanococcus vannielii
branched-chain amino acid aminotransferase	-	Thermomicrobium roseum
branched-chain amino acid aminotransferase	-	Thermobaculum terrenum
IlvE	-	Methanococcus vannielii
IlvE	-	Thermomicrobium roseum
IlvE	-	Thermobaculum terrenum
Mevan_0408	-	Methanococcus vannielii
trd_1610	-	Thermomicrobium roseum
Tter_1720	-	Thermobaculum terrenum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Thermomicrobium roseum
40	-	-	Thermobaculum terrenum
50	-	-	Methanococcus vannielii

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.2	-	(R)-1-phenylethylamine	pH 9.0, 50°C, recombinant enzyme, with 2-oxogluarate	Thermobaculum terrenum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	(S)-transamination with L-leucine	Methanococcus vannielii
8	-	(S)-transamination with L-leucine	Thermomicrobium roseum
8	-	(S)-transamination with L-leucine	Thermobaculum terrenum
9	-	(R)-transamination with (R)-phenylethylamine	Methanococcus vannielii
9	-	(R)-transamination with (R)-phenylethylamine	Thermomicrobium roseum
9	-	(R)-transamination with (R)-phenylethylamine	Thermobaculum terrenum

Cofactor

Cofactor	Comment	Organism
pyridoxal 5'-phosphate	PLP	Methanococcus vannielii
pyridoxal 5'-phosphate	PLP	Thermomicrobium roseum
pyridoxal 5'-phosphate	PLP	Thermobaculum terrenum

General Information

General Information	Comment	Organism
evolution	the enzyme belongs to the PLP fold type IV transaminases	Methanococcus vannielii
evolution	the enzyme belongs to the PLP fold type IV transaminases	Thermomicrobium roseum
evolution	the enzyme belongs to the PLP fold type IV transaminases	Thermobaculum terrenum

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.019	-	(R)-1-phenylethylamine	pH 9.0, 50°C, recombinant enzyme, with 2-oxogluarate	Thermobaculum terrenum