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Literature summary for 2.6.1.43 extracted from

  • Tyagi, R.K.; Datta, K.
    A rapid, high-yield purification of L-alanine:4,5-dioxovalerate transaminase from rat kidney mitochondria using an improved enzyme assay method (1994), Protein Expr. Purif., 5, 527-533.
    View publication on PubMed

General Stability

General Stability Organism
repeated freezing and thawing causes inactivation Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
hemin
-
Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion mitochondrial matrix Rattus norvegicus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
4 * 50000, mitochondrial isozyme, SDS-PAGE Rattus norvegicus
210000
-
gel filtration, gradient polyacrylamide gel electrophoresis Rattus norvegicus
225000
-
gel filtration, Sepharose 6B Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-alanine + 4,5-dioxopentanoate Rattus norvegicus alternate route for delta-aminolevulinate formation 5-aminolevulinate + pyruvate
-
ir
L-alanine + 4,5-dioxopentanoate Rattus norvegicus heme biosynthetic pathway 5-aminolevulinate + pyruvate
-
ir
L-alanine + 4,5-dioxopentanoate Rattus norvegicus Wistar alternate route for delta-aminolevulinate formation 5-aminolevulinate + pyruvate
-
ir
L-alanine + 4,5-dioxopentanoate Rattus norvegicus Wistar heme biosynthetic pathway 5-aminolevulinate + pyruvate
-
ir

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
Wistar
-
Rattus norvegicus Wistar
-
Wistar
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
36.9
-
-
Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, can be stored for nearly 1 year without significant loss of enzyme activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine + 4,5-dioxopentanoate
-
Rattus norvegicus 5-aminolevulinate + pyruvate
-
ir
L-alanine + 4,5-dioxopentanoate alternate route for delta-aminolevulinate formation Rattus norvegicus 5-aminolevulinate + pyruvate
-
ir
L-alanine + 4,5-dioxopentanoate heme biosynthetic pathway Rattus norvegicus 5-aminolevulinate + pyruvate
-
ir
L-alanine + 4,5-dioxopentanoate
-
Rattus norvegicus Wistar 5-aminolevulinate + pyruvate
-
ir
L-alanine + 4,5-dioxopentanoate alternate route for delta-aminolevulinate formation Rattus norvegicus Wistar 5-aminolevulinate + pyruvate
-
ir
L-alanine + 4,5-dioxopentanoate heme biosynthetic pathway Rattus norvegicus Wistar 5-aminolevulinate + pyruvate
-
ir

Subunits

Subunits Comment Organism
tetramer 4 * 50000, mitochondrial isozyme, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
EC 2.6.1.19 2-aminobutyrate aminotransferase and aminolevulinate transaminase activity reside in the same protein Rattus norvegicus
EC 2.6.1.40 dimethylarginine-pyruvate aminotransferase and D-3-aminoisobutyrate pyruvate aminotransferase activities resides in the same protein Rattus norvegicus
EC 2.6.1.44 alanine-glyoxylate transaminase and aminolevulinate transaminase activities reside in the same protein Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Rattus norvegicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 80 3fold increase of activity from 37°C to 60°C Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55 65 retains more than 90% maximum activity through 55-65°C, beyond 65°C activity decreases sharply Rattus norvegicus

pI Value

Organism Comment pI Value Maximum pI Value
Rattus norvegicus isoelectric focusing analysis, 2-dimensional gel electrophoresis
-
5