Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
wild-type and G82E mutant His-tagged enzyme expressed in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
G82E | natural enzyme variant. Significant reduction in affinty for pyridoxal 5'-phosphate and pyridoxamine 5'-phosphate. Mutant displays an altered conformational state of the bound pyridoxal 5'-phosphate and a decrease in overall catlytic activity to 0.1% of wild-type | Homo sapiens |
G82E | naturally occuring variant. Like the wild-type, the G82E variant is able to bind 2 mol pyridoxal 5'-phosphate/dimer, it exhibits a significant reduced affinity for pyridoxal 5'-phosphate and even more for pyridoxamine 5'-phosphate compared with wild-type, and an altered conformational state of the bound pyridoxal 5'-phosphate. Dramatic decrease of the overall catalytic activity (about 0.1% of that of normal alanine:glyoxylate aminotransferase), appears to be related to the inability to undergo an efficient transaldimination of the pyridoxal 5'-phosphate form of the enzyme with amino acids as well as an efficient conversion of AGT-pyridoxamine 5'-phosphate into AGT-pyridoxal 5'-phosphate | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-alanine | competitive inhibitor with L-alanine as varied substrate and glyoxylate as fixed substrate. Uncompetitive inhibitor with glyoxylate as varied substrate and L-alanine as fixed substrate; competitive with L-alanine, uncompetitive with glyoxylate | Homo sapiens | |
pyruvate | mixed type inhibition with L-alanine, competitive with glyoxylate; mixed type inhibitor with L-alanine as varied substrate and glyoxylate as fixed substrate. Competitive inhibitor with glyoxylate as varied substrate and L-alanine as fixed substrate | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
glyoxylate | mutant G82E, 25°C | Homo sapiens | |
0.15 | - |
glyoxylate | pH 7.4, 25°C, mutant enzyme G82E | Homo sapiens | |
0.21 | - |
pyruvate | pH 7.4, 25°C, wild-type enzyme | Homo sapiens | |
0.21 | - |
pyruvate | wild-type, 25°C | Homo sapiens | |
0.23 | - |
glyoxylate | pH 7.4, 25°C, wild-type enzyme | Homo sapiens | |
0.23 | - |
glyoxylate | wild-type, 25°C | Homo sapiens | |
15 | - |
L-alanine | mutant G82E, 25°C | Homo sapiens | |
15 | - |
L-alanine | pH 7.4, 25°C, mutant enzyme G82E | Homo sapiens | |
22 | - |
glycine | pH 7.4, 25°C, wild-type enzyme | Homo sapiens | |
22 | - |
glycine | wild-type, 25°C | Homo sapiens | |
31 | - |
L-alanine | pH 7.4, 25°C, wild-type enzyme | Homo sapiens | |
31 | - |
L-alanine | wild-type, 25°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + glyoxylate | Homo sapiens | the enzyme is highly specific for catalysing glyoxylate to glycine processing, thereby playing a key role in glyoxylate detoxification | pyruvate + glycine | - |
? | |
additional information | Homo sapiens | enzyme is highly specific for catalyzing glyoxylate to glycine processing, playing a key role in glyoxylate detoxification | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Homo sapiens | A2V838 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and G82E mutant His-tagged enzyme | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-alanine + glyoxylate = pyruvate + glycine | pyridoxamine 5'-phosphate remains bound to the enzyme during the catalytic cycle. The enzyme-pyridoxamine 5'-phosphate complex displays a reactivity towards oxo acids higher than that of the apo-enzyme in presence of pyridoxamine | Homo sapiens |
Storage Stability | Organism |
---|---|
-20°C, wild-type and mutant enzyme G82E are stable for at least 1 month | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycine + pyruvate | - |
Homo sapiens | L-alanine + glyoxylate | - |
? | |
L-alanine + glyoxylate | the enzyme is highly specific for catalysing glyoxylate to glycine processing, thereby playing a key role in glyoxylate detoxification | Homo sapiens | pyruvate + glycine | - |
? | |
L-alanine + glyoxylate | the enzyme is highly specific for catalysing glyoxylate to glycine processing | Homo sapiens | pyruvate + glycine | - |
? | |
L-alanine + glyoxylate | transamination half-reaction kinetic parameters | Homo sapiens | glycine + pyruvate | - |
r | |
L-arginine + pyruvate | - |
Homo sapiens | 5-guanidino-2-oxopentanoate + L-alanine | - |
? | |
L-asparagine + glyoxylate | - |
Homo sapiens | 4-amino-2,4-dioxobutanoate + glycine | - |
? | |
L-glutamate + glyoxylate | - |
Homo sapiens | 2-oxoglutarate + glycine | - |
? | |
L-phenylalanine + glyoxylate | - |
Homo sapiens | phenylpyruvate + glycine | - |
? | |
L-serine + glyoxylate | - |
Homo sapiens | 3-hydroxy-2-oxopropanoate + glycine | - |
? | |
additional information | enzyme is highly specific for catalyzing glyoxylate to glycine processing, playing a key role in glyoxylate detoxification | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AGT | - |
Homo sapiens |
alanine:glyoxylate aminotransferase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.068 | - |
glyoxylate | mutant G82E, 25°C | Homo sapiens | |
0.068 | - |
glyoxylate | pH 7.4, 25°C, mutant enzyme G82E | Homo sapiens | |
0.07 | - |
L-alanine | mutant G82E, 25°C | Homo sapiens | |
0.07 | - |
L-alanine | pH 7.4, 25°C, mutant enzyme G82E | Homo sapiens | |
0.33 | - |
glycine | pH 7.4, 25°C, wild-type enzyme | Homo sapiens | |
0.33 | - |
glycine | wild-type, 25°C | Homo sapiens | |
0.36 | - |
pyruvate | pH 7.4, 25°C, wild-type enzyme | Homo sapiens | |
0.36 | - |
pyruvate | wild-type, 25°C | Homo sapiens | |
45 | - |
glyoxylate | pH 7.4, 25°C, wild-type enzyme | Homo sapiens | |
45 | - |
L-alanine | pH 7.4, 25°C, wild-type enzyme | Homo sapiens | |
45 | - |
glyoxylate | wild-type, 25°C | Homo sapiens | |
45 | - |
L-alanine | wild-type, 25°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | like the wild-type, the G82E variant is able to bind 2 mol pyridoxal 5'-phosphate/dimer, it exhibits a significant reduced affinity for pyridoxal 5'-phosphate and even more for pyridoxamine 5'-phosphate compared with wild-type, and an altered conformational state of the bound pyridoxal 5'-phosphate | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2.3 | - |
pyruvate | glyoxylate as varied substrate and L-alanine as fixed substrate | Homo sapiens | |
14.3 | - |
D-alanine | with L-alanine as varied substrate and glyoxylate as fixed substrate, Kis | Homo sapiens | |
15.8 | - |
pyruvate | with L-alanine as varied substrate and glyoxylate as fixed substrate, Kis | Homo sapiens | |
22.8 | - |
pyruvate | with L-alanine as varied substrate and glyoxylate as fixed substrate, Kii | Homo sapiens | |
28.7 | - |
D-alanine | with glyoxylate as varied substrate and L-alanine as fixed substrate, Kii | Homo sapiens |