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Literature summary for 2.6.1.5 extracted from

  • Sobrado, V.R.; Montemartini-Kalisz, M.; Kalisz, H.M.; De La Fuente, M.C.; Hecht, H.J.; Nowicki, C.
    Involvement of conserved asparagine and arginine residues from the N-terminal region in the catalytic mechanism of rat liver and Trypanosoma cruzi tyrosine aminotransferases (2003), Protein Sci., 12, 1039-1050.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.5

Protein Variants

Protein Variants Comment Organism
N17S increase in Km-values, substantial decrease in kcat-values Trypanosoma cruzi
N54S reduced enzymic activity Rattus norvegicus
R20A increase in Km-values, substantial decrease in kcat-values Trypanosoma cruzi
R315K retains enzymic activity Rattus norvegicus
R417Q no enzymic activity Rattus norvegicus
R57A no enzymic activity Rattus norvegicus
R57Q no enzymic activity Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 2-oxoisocaproate mutant R20A, pH 7.5, 37°C Trypanosoma cruzi
0.35
-
 2-oxoglutarate mutant R315K, pH 7.5, 37°C Rattus norvegicus
0.4
-
 2-oxoglutarate wild-type, pH 7.5, 37°C Rattus norvegicus
0.6
-
 pyruvate mutant N17S, pH 7.5, 37°C Trypanosoma cruzi
0.6
-
 pyruvate mutant R20A, pH 7.5, 37°C Trypanosoma cruzi
0.8
-
 pyruvate wild-type, pH 7.5, 37°C Trypanosoma cruzi
0.9
-
 L-alanine wild-type, pH 7.5, 37°C Trypanosoma cruzi
1.2
-
 L-tyrosine wild-type, pH 7.5, 37°C Trypanosoma cruzi
1.2
-
 p-hydroxyphenylpyruvate wild-type, pH 7.5, 37°C Rattus norvegicus
1.2
-
 L-alanine mutant N17S, pH 7.5, 37°C Trypanosoma cruzi
1.2
-
 L-alanine mutant R20A, pH 7.5, 37°C Trypanosoma cruzi
1.2
-
 p-hydroxyphenylpyruvate mutant R315K, pH 7.5, 37°C Rattus norvegicus
1.3
-
 p-hydroxyphenylpyruvate mutant N54S, pH 7.5, 37°C Rattus norvegicus
1.9
-
 L-glutamate mutant R315K, pH 7.5, 37°C Rattus norvegicus
2.1
-
 L-tyrosine wild-type, pH 7.5, 37°C Rattus norvegicus
2.2
-
 L-glutamate wild-type, pH 7.5, 37°C Rattus norvegicus
2.3
-
 L-tyrosine mutant R20A, pH 7.5, 37°C Trypanosoma cruzi
2.3
-
 L-tyrosine mutant R315K, pH 7.5, 37°C Rattus norvegicus
2.4
-
 L-tyrosine mutant N17S, pH 7.5, 37°C Trypanosoma cruzi
2.8
-
 2-oxoisocaproate mutant N17S, pH 7.5, 37°C Trypanosoma cruzi
3.5
-
 2-oxoglutarate mutant N54S, pH 7.5, 37°C Rattus norvegicus
4.6
-
 2-oxoglutarate wild-type, pH 7.5, 37°C Trypanosoma cruzi
5.3
-
 L-tyrosine mutant N54S, pH 7.5, 37°C Rattus norvegicus
8.8
-
 L-glutamate mutant N54S, pH 7.5, 37°C Rattus norvegicus
9.6
-
 2-oxoglutarate mutant N17S, pH 7.5, 37°C Trypanosoma cruzi
14.8
-
 2-oxoglutarate mutant R20A, pH 7.5, 37°C Trypanosoma cruzi
20.4
-
 2-oxoisocaproate wild-type, pH 7.5, 37°C Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P04694
-
-
Trypanosoma cruzi P33447
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine + 2-oxoglutarate
-
 Trypanosoma cruzi pyruvate + L-glutamate
-
 ?
L-alanine + 2-oxoisocaproate
-
 Trypanosoma cruzi pyruvate + 2-aminoisocaproate
-
 ?
L-glutamate + 4-hydroxyphenylpyruvate
-
 Rattus norvegicus 2-oxoglutarate + L-tyrosine
-
 ?
L-tyrosine + 2-oxoglutarate
-
 Rattus norvegicus 4-hydroxyphenylpyruvate + L-glutamate
-
 ?
L-tyrosine + pyruvate
-
 Trypanosoma cruzi 4-hydroxyphenylpyruvate + L-alanine
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.35
-
 L-alanine mutant R20A, pH 7.5, 37°C Trypanosoma cruzi
0.4
-
 2-oxoisocaproate mutant R20A, pH 7.5, 37°C Trypanosoma cruzi
0.5
-
 pyruvate mutant R20A, pH 7.5, 37°C Trypanosoma cruzi
0.5
-
 2-oxoglutarate mutant R20A, pH 7.5, 37°C Trypanosoma cruzi
0.7
-
 L-tyrosine mutant R20A, pH 7.5, 37°C Trypanosoma cruzi
1.5
-
 pyruvate mutant N17S, pH 7.5, 37°C Trypanosoma cruzi
1.8
-
 L-tyrosine mutant N17S, pH 7.5, 37°C Trypanosoma cruzi
3.8
-
 L-alanine mutant N17S, pH 7.5, 37°C Trypanosoma cruzi
4.2
-
 2-oxoglutarate mutant N17S, pH 7.5, 37°C Trypanosoma cruzi
5.9
-
 p-hydroxyphenylpyruvate mutant N54S, pH 7.5, 37°C Rattus norvegicus
6.6
-
 L-glutamate mutant N54S, pH 7.5, 37°C Rattus norvegicus
9.3
-
 2-oxoisocaproate mutant N17S, pH 7.5, 37°C Trypanosoma cruzi
21.8
-
 2-oxoglutarate wild-type, pH 7.5, 37°C Trypanosoma cruzi
23.5
-
 L-alanine wild-type, pH 7.5, 37°C Trypanosoma cruzi
34
-
 2-oxoisocaproate wild-type, pH 7.5, 37°C Trypanosoma cruzi
44.6
-
 L-tyrosine wild-type, pH 7.5, 37°C Trypanosoma cruzi
47.4
-
 pyruvate wild-type, pH 7.5, 37°C Trypanosoma cruzi
116
-
 2-oxoglutarate mutant N54S, pH 7.5, 37°C Rattus norvegicus
132
-
 L-tyrosine mutant N54S, pH 7.5, 37°C Rattus norvegicus
155
-
 p-hydroxyphenylpyruvate mutant R315K, pH 7.5, 37°C Rattus norvegicus
165
-
 p-hydroxyphenylpyruvate wild-type, pH 7.5, 37°C Rattus norvegicus
199
-
 L-glutamate mutant R315K, pH 7.5, 37°C Rattus norvegicus
210
-
 L-glutamate wild-type, pH 7.5, 37°C Rattus norvegicus
490
-
 2-oxoglutarate mutant R315K, pH 7.5, 37°C Rattus norvegicus
510
-
 L-tyrosine mutant R315K, pH 7.5, 37°C Rattus norvegicus
566
-
 2-oxoglutarate wild-type, pH 7.5, 37°C Rattus norvegicus
576
-
 L-tyrosine wild-type, pH 7.5, 37°C Rattus norvegicus