KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
phenylpyruvate | cosubstrate L-tyrosine, pH 10.0, 30°C | Petunia x hybrida | |
1.8 | - |
2-oxoglutarate | cosubstrate phenylalanine, pH 10.0, 30°C | Petunia x hybrida | |
1.9 | - |
L-tyrosine | cosubstrate phenylpyruvate, pH 10.0, 30°C | Petunia x hybrida | |
2.6 | - |
phenylpyruvate | cosubstrate L-glutamate, pH 10.0, 30°C | Petunia x hybrida | |
4.3 | - |
L-phenylalanine | cosubstrate 4-hydroxyphenylpyruvate, pH 10.0, 30°C | Petunia x hybrida | |
7.9 | - |
4-hydroxyphenylpyruvate | cosubstrate phenylalanine, pH 10.0, 30°C | Petunia x hybrida | |
11.1 | - |
L-glutamate | cosubstrate phenylpyruvate, pH 10.0, 30°C | Petunia x hybrida | |
13.8 | - |
L-phenylalanine | cosubstrate 2-oxoglutarate, pH 10.0, 30°C | Petunia x hybrida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Petunia x hybrida | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Petunia x hybrida | V5M241 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + L-phenylalanine | - |
Petunia x hybrida | L-glutamate + phenylpyruvate | - |
? | |
4-hydroxyphenylpyruvate + L-phenylalanine | - |
Petunia x hybrida | L-glutamate + phenylpyruvate | - |
? | |
L-glutamate + phenylpyruvate | - |
Petunia x hybrida | 2-oxoglutarate + L-phenylalanine | - |
r | |
L-tyrosine + phenylpyruvate | - |
Petunia x hybrida | 4-hydroxyphenylpyruvate + L-phenylalanine | - |
r | |
additional information | activities with glutamate, methionine and leucine do not exceed 13% of the activity with tyrosine. Only phenylpyruvate and 2-oxoglutarate effectively serve as acceptors, and no activity is detected with prephenate | Petunia x hybrida | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PPY-AT | - |
Petunia x hybrida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
L-phenylalanine | cosubstrate 4-hydroxyphenylpyruvate, pH 10.0, 30°C | Petunia x hybrida | |
0.44 | - |
L-phenylalanine | cosubstrate 2-oxoglutarate, pH 10.0, 30°C | Petunia x hybrida | |
0.44 | - |
L-glutamate | cosubstrate phenylpyruvate, pH 10.0, 30°C | Petunia x hybrida | |
0.7 | - |
4-hydroxyphenylpyruvate | cosubstrate phenylalanine, pH 10.0, 30°C | Petunia x hybrida | |
0.92 | - |
2-oxoglutarate | cosubstrate phenylalanine, pH 10.0, 30°C | Petunia x hybrida | |
1.19 | - |
phenylpyruvate | cosubstrate L-glutamate, pH 10.0, 30°C | Petunia x hybrida | |
1.46 | - |
phenylpyruvate | cosubstrate L-tyrosine, pH 10.0, 30°C | Petunia x hybrida | |
1.48 | - |
L-tyrosine | cosubstrate phenylpyruvate, pH 10.0, 30°C | Petunia x hybrida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Petunia x hybrida |
General Information | Comment | Organism |
---|---|---|
physiological function | Petunia hybrida utilizes a microbial-like phenylpyruvate pathway to produce phenylalanine, and flux through this route is increased when the entry point to the arogenate pathway is limiting. The plant phenylpyruvate pathway utilizes cytosolic tyrosine aminotransferase that links the coordinated catabolism of tyrosine to serve as the amino donor. The enzyme is able to functionally complement the Escherichia coli phenylalanine auxotrophic mutant DL39 which lacks the three aminotransferases, AspC (aspartate aminotransferase), TyrB (L-Tyrosine aminotransferase) and IlvE (branched-chain aminotransferase) | Petunia x hybrida |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
L-phenylalanine | cosubstrate 2-oxoglutarate, pH 10.0, 30°C | Petunia x hybrida | |
0.04 | - |
L-phenylalanine | cosubstrate 4-hydroxyphenylpyruvate, pH 10.0, 30°C | Petunia x hybrida | |
0.04 | - |
L-glutamate | cosubstrate phenylpyruvate, pH 10.0, 30°C | Petunia x hybrida | |
0.09 | - |
4-hydroxyphenylpyruvate | cosubstrate phenylalanine, pH 10.0, 30°C | Petunia x hybrida | |
0.46 | - |
phenylpyruvate | cosubstrate L-glutamate, pH 10.0, 30°C | Petunia x hybrida | |
0.51 | - |
2-oxoglutarate | cosubstrate phenylalanine, pH 10.0, 30°C | Petunia x hybrida | |
0.77 | - |
L-tyrosine | cosubstrate phenylpyruvate, pH 10.0, 30°C | Petunia x hybrida | |
0.96 | - |
phenylpyruvate | cosubstrate L-tyrosine, pH 10.0, 30°C | Petunia x hybrida |