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Literature summary for 2.6.1.5 extracted from

  • Sasidharan, S.; Saudagar, P.
    Mapping N- and C-terminals of Leishmania donovani tyrosine aminotransferase by gene truncation strategy a functional study using in vitro and in silico approaches (2020), Sci. Rep., 10, 12463 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.5

General Stability

General Stability Organism
activity of the N-terminal domain in the presence of urea is recorded till 2.5 M urea, after which negligible activity is recorded till 8 M urea concentration. In the case of the C-terminal domain, the activity of the protein gradually decreases till 2 M urea, and no further activity is detected from 3 M urea concentration. Leishmania donovani

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.468
-
 L-tyrosine N-terminal domain, with pyruvate as cosubstrate, at pH 8.0 and 37°C Leishmania donovani
4.581
-
 L-tyrosine C-terminal domain, with pyruvate as cosubstrate, at pH 8.0 and 37°C Leishmania donovani

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tyrosine + 2-oxoglutarate Leishmania donovani
-
 4-hydroxyphenylpyruvate + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Leishmania donovani A0A5B8TZA8
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Leishmania donovani

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tyrosine + 2-oxoglutarate
-
 Leishmania donovani 4-hydroxyphenylpyruvate + L-glutamate
-
 ?
L-tyrosine + pyruvate
-
 Leishmania donovani 4-hydroxyphenylpyruvate + L-alanine
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, N-terminal domain, SDS-PAGE Leishmania donovani
? x * 45000, C-terminal domain, SDS-PAGE Leishmania donovani

Synonyms

Synonyms Comment Organism
L-tyrosine: 2 oxoglutarate aminotransferase
-
 Leishmania donovani
TAT
-
 Leishmania donovani
TATase
-
 Leishmania donovani
tyrosine aminotransferase
-
 Leishmania donovani

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70 the activity of the full-length enzyme as well as its truncated N-terminal domain remains stable from 20-70°C, and then gradually decreases until 100°C. The truncated C-terminal domain displays its highest activity at 30-40°C but the activity decreases drastically at temperatures higher than 40°C Leishmania donovani

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Leishmania donovani

pH Range

pH Minimum pH Maximum Comment Organism
4 9 more than 50% activity between pH 4.0 and 9.0 Leishmania donovani

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Leishmania donovani