Literature summary for 2.6.1.50 extracted from

Ahlert, J.; Distler, J.; Mansouri, K.; Piepersberg, W.
Identification of stsC, the gene encoding the L-glutamine:scyllo-inosose aminotransferase from streptomycin-producing Streptomycetes (1997), Arch. Microbiol., 168, 102-113.

Search for more literature for 2.6.1.50

Cloned(Commentary)

Cloned (Comment)	Organism
gene StsC identified, cloned by PCR and expressed in Escherichia coli BL21 and Streptomyces lividans	Streptomyces griseus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
45000	-	2 * 45000, SDS-PAGE	Streptomyces griseus
45500	-	SDS-PAGE	Streptomyces griseus
95000	100000	gel filtration	Streptomyces griseus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone	Streptomyces bikiniensis	-	2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol	-	r
L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone	Streptomyces hygroscopicus	-	2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol	-	r
L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone	Streptomyces griseus	streptidine subpathway of streptomycin biosynthesis	2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol	-	r
L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone	Streptomyces bikiniensis NRRL 1049b	-	2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol	-	r

Organism

Organism	UniProt	Comment	Textmining
no activity in Escherichia coli	-	host strain, free of enzymatic activity	-
no activity in Streptomyces lividans	-	66 strain TK23, host strains, free of enzymatic activity	-
no activity in Streptomyces lividans	-	66 strain 1326	-
Streptomyces bikiniensis	-	NRRL 1049b	-
Streptomyces bikiniensis NRRL 1049b	-	NRRL 1049b	-
Streptomyces griseus	-	N2-3-11, DSM 40236	-
Streptomyces hygroscopicus	-	subsp. glebosus DSM 40823	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme, pJAW76-2, expressed in Streptomyces lividans	Streptomyces griseus

Storage Stability

Storage Stability	Organism
6°C, purified enzyme stable for several weeks	Streptomyces griseus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone	-	Streptomyces griseus	2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol	-	r
L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone	-	Streptomyces bikiniensis	2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol	-	r
L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone	-	Streptomyces hygroscopicus	2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol	-	r
L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone	streptidine subpathway of streptomycin biosynthesis	Streptomyces griseus	2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol	-	r
L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone	-	Streptomyces bikiniensis NRRL 1049b	2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol	-	r
L-glutamine + scyllo-inosose	-	Streptomyces griseus	scyllo-inosamine + 2-oxoglutaramate	spontaneously cyclization of 2-oxoglutaramate to 2-pyrrolidone-5-hydroxy-5-carboxylic acid	r

Subunits

Subunits	Comment	Organism
dimer	2 * 45000, SDS-PAGE	Streptomyces griseus

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Release 2023.1 (January 2023)