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Literature summary for 2.6.1.52 extracted from

  • Yang, C.; Stampouloglou, E.; Kingston, N.; Zhang, L.; Monti, S.; Varelas, X.
    Glutamine-utilizing transaminases are a metabolic vulnerability of TAZ/YAP-activated cancer cells (2018), EMBO Rep., 19, e43577 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.52

Cloned(Commentary)

Cloned (Comment) Organism
quantitative real-time PCR enzyme expression analysis Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Aminooxyacetate AOA, the transaminase inhibitor represses cell growth in a TAZ/YAP-dependent manner Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
O-phospho-L-serine + 2-oxoglutarate Homo sapiens
-
 3-phosphooxypyruvate + L-glutamate
-
 r

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9Y617
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from rat brain by ultracentrifugation at 220000 x g, followed by ammonium sulfate fractionation, hydropbobic interaction chromatography, anion exchange chromatography, and hydroxyapatite chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
breast cancer cell transcriptional regulators TAZ/YAP activity positively correlates with transaminase expression in breast cancer patients Homo sapiens
-
BT-474 cell
-
 Homo sapiens
-
HCC-38 cell
-
 Homo sapiens
-
HMT-3522 S1 cell
-
 Homo sapiens
-
MDA-MB-231 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O-phospho-L-serine + 2-oxoglutarate
-
 Homo sapiens 3-phosphooxypyruvate + L-glutamate
-
 r

Synonyms

Synonyms Comment Organism
phosphoserine aminotransferase
-
 Homo sapiens
PSAT1
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Homo sapiens

Expression

Organism Comment Expression
Homo sapiens GOT1 expression level is reduced in TAZ/YAP siRNA knockout cells. The inhibition of transamination preferentially suppresses those breast cancer cells that express high levels of TAZ/YAP (e.g. MDA-MB-231 and HCC38 cells ), not TAZ/YAP-low-level expressing cell lines (e.g. BT474 and HMT-3522 S1) down
Homo sapiens transcriptional regulators TAZ and YAP (TAZ/YAP) promote glutamine dependence in breast cancer cells and activate the expression of glutamine-utilizing transaminases to support cell growth. TAZ/YAP induce glutamic-oxaloacetic transaminase (GOT1) and phosphoserine aminotransferase (PSAT1) expression. TAZ and YAP are required for glutamine-utilizing transaminase expression in breast cancer cells up

General Information

General Information Comment Organism
metabolism transcriptional regulators TAZ and YAP (TAZ/YAP) promote glutamine dependence in breast cancer cells and activate the expression of glutamine-utilizing transaminases to support cell growth. TAZ and YAP induce glutamic-oxaloacetic transaminase (GOT1, EC 2.6.1.64) and phosphoserine aminotransferase (PSAT1) expression. Transcriptional regulators TAZ/YAP activity positively correlates with transaminase expression in breast cancer patients, while transaminase inhibitor aminooxyacetate (AOA) represses cell growth in a TAZ/YAP-dependent manner. Thus, transamination is a potential vulnerable metabolic requirement for TAZ/YAP-driven breast cancer Homo sapiens