Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.6.1.57 extracted from

  • Dindo, M.; Costanzi, E.; Pieroni, M.; Costantini, C.; Annunziato, G.; Bruno, A.; Keller, N.P.; Romani, L.; Zelante, T.; Cellini, B.
    Biochemical characterization of Aspergillus fumigatus AroH, a putative aromatic amino acid aminotransferase (2018), Front. Mol. Biosci., 5, 104 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Aspergillus fumigatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
alpha-aminoadipate pH 7.4, 25°C, recombinant enzyme, with cosubstrate L-glutamate Aspergillus fumigatus
0.18
-
2-oxoglutarate pH 7.4, 25°C, recombinant enzyme, with cosubstrate L-tryptophan Aspergillus fumigatus
0.5
-
L-tyrosine pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate Aspergillus fumigatus
1.4
-
L-phenylalanine pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate Aspergillus fumigatus
2.1
-
L-tryptophan pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate Aspergillus fumigatus
3.3
-
alpha-aminoadipate pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate Aspergillus fumigatus
9
-
L-kynurenine pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate Aspergillus fumigatus
10
-
L-glutamate pH 7.4, 25°C, recombinant enzyme, with cosubstrate alpha-aminoadipate Aspergillus fumigatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
107000
-
recombinant enzyme, gel filtration Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
an aromatic amino acid + 2-oxoglutarate Aspergillus fumigatus
-
an aromatic oxo acid + L-glutamate
-
r

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + L-phenylalanine
-
Aspergillus fumigatus L-glutamate + phenylpyruvate
-
r
2-oxoglutarate + L-tryptophan
-
Aspergillus fumigatus L-glutamate + 3-indole-2-oxopropanoate
-
r
2-oxoglutarate + L-tyrosine
-
Aspergillus fumigatus L-glutamate + 4-hydroxyphenylpyruvate
-
r
alpha-aminoadipate + 2-oxoglutarate
-
Aspergillus fumigatus 2-oxoadipate + L-glutamate
-
r
alpha-aminoadipate + L-glutamate
-
Aspergillus fumigatus 2-oxoglutarate + ?
-
r
an aromatic amino acid + 2-oxoglutarate
-
Aspergillus fumigatus an aromatic oxo acid + L-glutamate
-
r
L-kynurenine + 2-oxoglutarate reaction of EC 2.6.1.7 Aspergillus fumigatus 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate
-
r
additional information AroH behaves as an aromatic amino acid aminotransferase, but also accepts L-kynurenine and alpha-aminoadipate as amino donors Aspergillus fumigatus ?
-
-

Subunits

Subunits Comment Organism
homodimer 2 * 59480, sequence calculation, 2 * 57000, recombinant enzyme, SDS-PAGE Aspergillus fumigatus

Synonyms

Synonyms Comment Organism
AroH
-
Aspergillus fumigatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
Aspergillus fumigatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5
-
alpha-aminoadipate pH 7.4, 25°C, recombinant enzyme, with cosubstrate L-glutamate Aspergillus fumigatus
3
-
L-tryptophan pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate Aspergillus fumigatus
4
-
L-tyrosine pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate Aspergillus fumigatus
4.1
-
L-kynurenine pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate Aspergillus fumigatus
7.4
-
L-phenylalanine pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate Aspergillus fumigatus
10.3
-
L-glutamate pH 7.4, 25°C, recombinant enzyme, with cosubstrate alpha-aminoadipate Aspergillus fumigatus
12.5
-
2-oxoglutarate pH 7.4, 25°C, recombinant enzyme, with cosubstrate L-tryptophan Aspergillus fumigatus
13.2
-
alpha-aminoadipate pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate Aspergillus fumigatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
Aspergillus fumigatus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate PLP, the enzyme binds the coenzyme forming a deprotonated internal aldimine in equilibrium with a protonated ketoenamine form Aspergillus fumigatus

General Information

General Information Comment Organism
additional information homology modeling of the AroH structure using the structure of Saccharomyces cerevisiae Aro8 (PDB ID 4JE5) as template Aspergillus fumigatus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.4
-
L-kynurenine pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate Aspergillus fumigatus
0.96
-
L-glutamate pH 7.4, 25°C, recombinant enzyme, with cosubstrate alpha-aminoadipate Aspergillus fumigatus
1.4
-
L-tryptophan pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate Aspergillus fumigatus
4
-
alpha-aminoadipate pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate Aspergillus fumigatus
5.2
-
L-phenylalanine pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate Aspergillus fumigatus
8
-
L-tyrosine pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate Aspergillus fumigatus
14
-
alpha-aminoadipate pH 7.4, 25°C, recombinant enzyme, with cosubstrate L-glutamate Aspergillus fumigatus
90
-
2-oxoglutarate pH 7.4, 25°C, recombinant enzyme, with cosubstrate L-tryptophan Aspergillus fumigatus