Cloned (Comment) | Organism |
---|---|
recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Aspergillus fumigatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
alpha-aminoadipate | pH 7.4, 25°C, recombinant enzyme, with cosubstrate L-glutamate | Aspergillus fumigatus | |
0.18 | - |
2-oxoglutarate | pH 7.4, 25°C, recombinant enzyme, with cosubstrate L-tryptophan | Aspergillus fumigatus | |
0.5 | - |
L-tyrosine | pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate | Aspergillus fumigatus | |
1.4 | - |
L-phenylalanine | pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate | Aspergillus fumigatus | |
2.1 | - |
L-tryptophan | pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate | Aspergillus fumigatus | |
3.3 | - |
alpha-aminoadipate | pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate | Aspergillus fumigatus | |
9 | - |
L-kynurenine | pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate | Aspergillus fumigatus | |
10 | - |
L-glutamate | pH 7.4, 25°C, recombinant enzyme, with cosubstrate alpha-aminoadipate | Aspergillus fumigatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
107000 | - |
recombinant enzyme, gel filtration | Aspergillus fumigatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
an aromatic amino acid + 2-oxoglutarate | Aspergillus fumigatus | - |
an aromatic oxo acid + L-glutamate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + L-phenylalanine | - |
Aspergillus fumigatus | L-glutamate + phenylpyruvate | - |
r | |
2-oxoglutarate + L-tryptophan | - |
Aspergillus fumigatus | L-glutamate + 3-indole-2-oxopropanoate | - |
r | |
2-oxoglutarate + L-tyrosine | - |
Aspergillus fumigatus | L-glutamate + 4-hydroxyphenylpyruvate | - |
r | |
alpha-aminoadipate + 2-oxoglutarate | - |
Aspergillus fumigatus | 2-oxoadipate + L-glutamate | - |
r | |
alpha-aminoadipate + L-glutamate | - |
Aspergillus fumigatus | 2-oxoglutarate + ? | - |
r | |
an aromatic amino acid + 2-oxoglutarate | - |
Aspergillus fumigatus | an aromatic oxo acid + L-glutamate | - |
r | |
L-kynurenine + 2-oxoglutarate | reaction of EC 2.6.1.7 | Aspergillus fumigatus | 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate | - |
r | |
additional information | AroH behaves as an aromatic amino acid aminotransferase, but also accepts L-kynurenine and alpha-aminoadipate as amino donors | Aspergillus fumigatus | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 59480, sequence calculation, 2 * 57000, recombinant enzyme, SDS-PAGE | Aspergillus fumigatus |
Synonyms | Comment | Organism |
---|---|---|
AroH | - |
Aspergillus fumigatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Aspergillus fumigatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | - |
alpha-aminoadipate | pH 7.4, 25°C, recombinant enzyme, with cosubstrate L-glutamate | Aspergillus fumigatus | |
3 | - |
L-tryptophan | pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate | Aspergillus fumigatus | |
4 | - |
L-tyrosine | pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate | Aspergillus fumigatus | |
4.1 | - |
L-kynurenine | pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate | Aspergillus fumigatus | |
7.4 | - |
L-phenylalanine | pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate | Aspergillus fumigatus | |
10.3 | - |
L-glutamate | pH 7.4, 25°C, recombinant enzyme, with cosubstrate alpha-aminoadipate | Aspergillus fumigatus | |
12.5 | - |
2-oxoglutarate | pH 7.4, 25°C, recombinant enzyme, with cosubstrate L-tryptophan | Aspergillus fumigatus | |
13.2 | - |
alpha-aminoadipate | pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate | Aspergillus fumigatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Aspergillus fumigatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | PLP, the enzyme binds the coenzyme forming a deprotonated internal aldimine in equilibrium with a protonated ketoenamine form | Aspergillus fumigatus |
General Information | Comment | Organism |
---|---|---|
additional information | homology modeling of the AroH structure using the structure of Saccharomyces cerevisiae Aro8 (PDB ID 4JE5) as template | Aspergillus fumigatus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
L-kynurenine | pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate | Aspergillus fumigatus | |
0.96 | - |
L-glutamate | pH 7.4, 25°C, recombinant enzyme, with cosubstrate alpha-aminoadipate | Aspergillus fumigatus | |
1.4 | - |
L-tryptophan | pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate | Aspergillus fumigatus | |
4 | - |
alpha-aminoadipate | pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate | Aspergillus fumigatus | |
5.2 | - |
L-phenylalanine | pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate | Aspergillus fumigatus | |
8 | - |
L-tyrosine | pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate | Aspergillus fumigatus | |
14 | - |
alpha-aminoadipate | pH 7.4, 25°C, recombinant enzyme, with cosubstrate L-glutamate | Aspergillus fumigatus | |
90 | - |
2-oxoglutarate | pH 7.4, 25°C, recombinant enzyme, with cosubstrate L-tryptophan | Aspergillus fumigatus |