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Literature summary for 2.6.1.59 extracted from
- Characterization and investigation of substrate specificity of the sugar aminotransferase WecE from E. coli K12 (2004), Chem. Biol., 11, 915-925.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21 | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (aminooxy)acetic acid | - |
Escherichia coli |  |
| gabaculine | - |
Escherichia coli | |
| hydroxylamine | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
dTDP-4-dehydro-6-deoxy-D-glucose | pH 7.5, 25°C | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
4 * 44000, SDS-PAGE | Escherichia coli |
| 180000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P27833 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate | - |
Escherichia coli | dTDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutarate | - |
? | |
| dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamine | 65% of the activity with L-glutamate | Escherichia coli | dTDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutaramate | - |
? | |
| dTDP-4-dehydro-6-deoxy-D-mannose + L-glutamate | - |
Escherichia coli | ? + 2-oxoglutarate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 44000, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| WecE | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Escherichia coli |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 45 | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.38 | - |
dTDP-4-dehydro-6-deoxy-D-glucose | pH 7.5, 25°C | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | - |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Escherichia coli | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.62 | - |
dTDP-4-dehydro-6-deoxy-D-glucose | pH 7.5, 25°C | Escherichia coli | |
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