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Literature summary for 2.6.1.6 extracted from

  • Aki, K.; Ogawa, K.; Ichihara, A.
    Transaminases of branched chain amino acids. IV. Purification and properties of two enzymes from rat liver (1968), Biochim. Biophys. Acta, 159, 276-284.
    View publication on PubMed
Search for more literature for 2.6.1.6

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol slight inhibition Rattus norvegicus
p-chloromercuribenzoate 1.0 mM, complete inhibition Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.065
-
 2-oxoglutarate pH 8.2, 37°C Rattus norvegicus
25
-
 leucine pH 8.2, 37°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm predominantly found in mitochondrion Rattus norvegicus 5737
-
microsome
-
 Rattus norvegicus
-
-
mitochondrion
-
 Rattus norvegicus 5739
-
nucleus
-
 Rattus norvegicus 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-leucine + 2-oxoglutarate Rattus norvegicus leucine metabolism and hence of cholesterogenesis and ketogenesis, not present in fetal rat liver, but after birth activity appears and increases rapidly 4-methyl-2-oxopentanoate + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.033
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-leucine + 2-oxoglutarate
-
 Rattus norvegicus 4-methyl-2-oxopentanoate + L-glutamate
-
 r
L-leucine + 2-oxoglutarate leucine metabolism and hence of cholesterogenesis and ketogenesis, not present in fetal rat liver, but after birth activity appears and increases rapidly Rattus norvegicus 4-methyl-2-oxopentanoate + L-glutamate
-
 ?
L-leucine + oxaloacetate
-
 Rattus norvegicus 4-methyl-2-oxopentanoate + L-aspartate
-
 ?
additional information specific for leucine, isoleucine or valine, no other amino acid would serve as an amino donor, pyruvate is no amino acid acceptor Rattus norvegicus ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.7
-
-
 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate Km 0.004 mM Rattus norvegicus