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Literature summary for 2.6.1.64 extracted from

  • Cooper, A.J.L.; Meister, A.
    Isolation and properties of a new glutamine transaminase form rat kidney (1974), J. Biol. Chem., 249, 2554-2561.
    View publication on PubMed
Search for more literature for 2.6.1.64

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 2-Oxoglutaramate pH 8.5, 37°C, L-phenylalanine as amino group donor Rattus norvegicus
0.58
-
 L-phenylalanine pH 8.5, 37°C Rattus norvegicus
0.92
-
 alpha-keto-gamma-methiolbutyrate pH 8.5, 37°C, L-phenylalanine as amino group donor Rattus norvegicus
1.4
-
 L-glutamine pH 8.5, 37°C, phenylpyruvate Rattus norvegicus
4.2
-
 L-methionine pH 8.5, 37°C, phenylpyruvate Rattus norvegicus
4.4
-
 L-ethionine pH 8.5, 37°C, phenylpyruvate Rattus norvegicus
4.8
-
 glyoxylate pH 8.5, 37°C Rattus norvegicus
7.8
-
 alpha-keto-n-valerate pH 8.5, 37°C, L-phenylalanine as amino group donor Rattus norvegicus
8.7
-
 alpha-keto-n-caproate pH 8.5, 37°C, L-phenylalanine as amino group donor Rattus norvegicus
18
-
 alpha-Ketobutyrate pH 8.5, 37°C Rattus norvegicus
27
-
 pyruvate pH 8.5, 37°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Rattus norvegicus 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 2 * 55000, liver enzyme Rattus norvegicus
110000
-
 kidney enzyme, gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 Sprague-Dawley
-
Rattus norvegicus Sprague-Dawley
-
 Sprague-Dawley
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-
cardiac muscle
-
 Rattus norvegicus
-
kidney
-
 Rattus norvegicus
-
liver
-
 Rattus norvegicus
-
additional information no glutamine transaminase activity in skeletal muscle, testis, spleen, pancreas, lung and intestine Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.68
-
 cytoplasmic isozyme Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-cyano-L-alpha-aminobutyrate + glyoxylate
-
 Rattus norvegicus 4-cyano-2-oxobutanoate + glycine
-
 r
4-cyano-L-alpha-aminobutyrate + phenylpyruvate
-
 Rattus norvegicus 4-cyano-2-oxobutanoate + L-phenylalanine
-
 r
albizziin + glyoxylate
-
 Rattus norvegicus 3-carbamido-2-oxopropanoate + glycine
-
 r
DL-homoserine + glyoxylate
-
 Rattus norvegicus 4-hydroxy-2-oxobutanoate + glycine
-
 r
DL-homoserine + phenylpyruvate
-
 Rattus norvegicus 4-hydroxy-2-oxobutanoate + phenylalanine
-
 r
L-cysteine + glyoxylate
-
 Rattus norvegicus 3-mercapto-2-oxopropanoate + glycine
-
 r
L-cysteine + phenylpyruvate
-
 Rattus norvegicus 3-mercapto-2-oxopropanoate + L-phenylalanine
-
 r
L-ethionine + glyoxylate
-
 Rattus norvegicus 4-ethylsulfanyl-2-oxobutanoate + glycine
-
 r
L-ethionine + phenylpyruvate
-
 Rattus norvegicus 4-ethylsulfanyl-2-oxobutanoate + L-phenylalanine
-
 r
L-gamma-glutamylhydrazone of glyoxylate + glyoxylate
-
 Rattus norvegicus ?
-
 r
L-gamma-glutamylhydrazone of glyoxylate + phenylpyruvate
-
 Rattus norvegicus ?
-
 r
L-gamma-glutamylhydrazone of phenylpyruvate + glyoxylate
-
 Rattus norvegicus ?
-
 r
L-gamma-glutamylhydrazone of phenylpyruvate + phenylpyruvate
-
 Rattus norvegicus ?
-
 r
L-glutamic acid-gamma-methyl ester + glyoxylate liver enzyme L Rattus norvegicus 2-oxo-pentandioate-5-methyl ester + glycine
-
 r
L-glutamic acid-gamma-methyl ester + phenylpyruvate liver enzyme L Rattus norvegicus 2-oxo-pentandioate-5-methyl ester + L-phenylalanine
-
 r
L-glutamine + 2-oxobutyrate
-
 Rattus norvegicus 2-oxoglutaramate + 2-aminobutanoate
-
 r
L-glutamine + 2-oxoglutaramate
-
 Rattus norvegicus 2-oxoglutaramate + L-glutamine
-
 r
L-glutamine + 2-oxoglutarate
-
 Rattus norvegicus 2-oxoglutaramate + L-glutamate
-
 r
L-glutamine + 2-oxovalerate
-
 Rattus norvegicus 2-oxoglutaramate + L-norvaline
-
 r
L-glutamine + 3-hydroxypyruvate
-
 Rattus norvegicus 2-oxoglutaramate + L-serine
-
 r
L-glutamine + alpha-keto-delta-carbamidovalerate
-
 Rattus norvegicus 2-oxoglutaramate + L-arginine
-
 r
L-glutamine + alpha-keto-n-caproate
-
 Rattus norvegicus 2-oxoglutaramate + L-norleucine
-
 r
L-glutamine + alpha-ketoisocaproate
-
 Rattus norvegicus 2-oxoglutaramate + L-leucine
-
 r
L-glutamine + alpha-ketoisovalerate
-
 Rattus norvegicus 2-oxoglutaramate + L-valine
-
 r
L-glutamine + glyoxylate isozyme L highly active, isozyme K relatively little activity toward glyoxylate Rattus norvegicus 2-oxoglutaramate + glycine
-
 r
L-glutamine + oxaloacetate
-
 Rattus norvegicus 2-oxoglutaramate + L-aspartate
-
 r
L-glutamine + p-hydroxyphenylpyruvate
-
 Rattus norvegicus 2-oxoglutaramate + L-tyrosine
-
 r
L-glutamine + phenylpyruvate
-
 Rattus norvegicus 2-oxoglutaramate + L-phenylalanine
-
 r
L-glutamine + pyruvate isozyme L highly active, isozyme K relatively little activity toward pyruvate Rattus norvegicus 2-oxoglutaramate + L-alanine
-
 r
L-glutamylmethylamide + glyoxylate liver enzyme L Rattus norvegicus 2-oxoglutarate-5-methylamide + glycine
-
 r
L-glutamylmethylamide + phenylpyruvate
-
 Rattus norvegicus 2-oxoglutarate-5-methylamide + L-phenylalanine
-
 r
L-histidine + pyruvate poor substrates Rattus norvegicus 3-(1H-imidazol-4-yl)-2-oxopropanoate + L-alanine
-
 r
L-methionine + 2-oxoglutaramate isozymes L and K highly active Rattus norvegicus L-glutamine + 4-methylsulfanyl-2-oxobutanoate
-
 r
L-methionine + glyoxylate
-
 Rattus norvegicus 4-methylsulfanyl-2-oxobutanoate + glycine
-
 r
L-methionine + phenylpyruvate
-
 Rattus norvegicus 4-methylsulfanyl-2-oxobutanoate + L-phenylalanine
-
 r
L-methionine sulfone + glyoxylate
-
 Rattus norvegicus 4-methylsulfonyl-2-oxobutanoate + glycine
-
 r
L-methionine sulfone + phenylpyruvate
-
 Rattus norvegicus 4-methylsulfonyl-2-oxobutanoate + L-phenylalanine
-
 r
L-methionine-SR-sulfoxide + glyoxylate
-
 Rattus norvegicus 4-methylsulfinyl-2-oxobutanoate + glycine
-
 r
L-methionine-SR-sulfoxide + phenylpyruvate
-
 Rattus norvegicus 4-methylsulfinyl-2-oxobutanoate + L-phenylalanine
-
 r
L-methionine-SR-sulfoximine + glyoxylate
-
 Rattus norvegicus 4-methylsulfanyl-2-oxobutanoate-SR-sulfoximine + glycine
-
 r
L-methionine-SR-sulfoximine + phenylpyruvate
-
 Rattus norvegicus 4-methylsulfanyl-2-oxobutanoate-SR-sulfoximine + L-phenylalanine
-
 r
L-phenylalanine + 2-oxoglutaramate
-
 Rattus norvegicus phenylpyruvate + L-glutamine
-
 r
L-phenylalanine + 4-(methylsulfanyl)-2-oxobutanoate
-
 Rattus norvegicus phenylpyruvate + L-methionine
-
 r
L-phenylalanine + 4-methylsulfanyl-2-oxobutanoate isozyme K very active toward phenylalanine Rattus norvegicus phenylpyruvate + L-methionine
-
 r
L-phenylalanine + alpha-keto-n-caproate
-
 Rattus norvegicus phenylpyruvate + L-norleucine
-
 r
L-phenylalanine + alpha-keto-n-valerate
-
 Rattus norvegicus phenylpyruvate + L-norvaline
-
 r
L-phenylalanine + alpha-ketobutyrate
-
 Rattus norvegicus phenylpyruvate + 2-aminobutyrate
-
 r
L-phenylalanine + glyoxylate
-
 Rattus norvegicus phenylpyruvate + glycine
-
 r
L-phenylalanine + pyruvate
-
 Rattus norvegicus phenylpyruvate + L-alanine
-
 r
L-tyrosine + 2-oxoglutaramate
-
 Rattus norvegicus 3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamine
-
 r
additional information 4 separate isozymes, cytoplasmic and mitochondrial, in kidney and liver, trimethylpyruvate and D and L-alpha-keto-beta-methylvalerate are no amino acceptors, L-gamma-glutamyldimethylamide is no substrate Rattus norvegicus ?
-
 ?
O-carbamoyl-L-serine + glyoxylate
-
 Rattus norvegicus O-carbamoyl-(3-hydroxy-2-oxo-propanoate) + glycine
-
 r
S-carbamoyl-L-cysteine + glyoxylate
-
 Rattus norvegicus S-carbamoyl-3-mercapto-2-oxo-propanoate + glycine
-
 r
S-methyl-L-cysteine + glyoxylate
-
 Rattus norvegicus 3-methylsulfanyl-2-oxopropanoate + glycine
-
 r
S-methyl-L-cysteine + phenylpyruvate
-
 Rattus norvegicus 3-methylsulfanyl-2-oxopropanoate + L-phenylalanine
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 55000, liver enzyme Rattus norvegicus

Synonyms

Synonyms Comment Organism
glutamine transaminase K
-
 Rattus norvegicus
glutamine transaminase L
-
 Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 liver glutaminase transaminase L is unstable at 60°C in absence of an added alpha-keto-acid, kidney glutamine transaminase L is stable Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Rattus norvegicus