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Literature summary for 2.6.1.65 extracted from

  • Bozler, G.; Robertson, J.M.; Ohsugi, M.; Hensley, C.; Barker, H.A.
    Metabolism of L-beta-lysine in a Pseudomonas: conversion of 6-N-acetyl-L-beta-lysine to 3-keto-6-acetamidohexanoate and of 4-aminobutyrate to succinic semialdehyde by different transaminases (1979), Arch. Biochem. Biophys., 197, 226-235.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5
-
 2-oxoglutarate 35°C Pseudomonas sp.
5
-
 6-Acetamido-3-aminohexanoate 35°C Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-acetamido-3-aminohexanoate + 2-oxoglutarate Pseudomonas sp. inducible enzyme of lysine catabolism 6-acetamido-3-oxohexanoate + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 B4
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.3 0.5 crude enzyme extract, 35°C Pseudomonas sp.

Storage Stability

Storage Stability Organism
-20°C, about 5% loss of activity per month Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-acetamido-3-aminohexanoate + 2-oxoglutarate i.e. 6-N-acetyl-L-beta-lysine Pseudomonas sp. 6-acetamido-3-oxohexanoate + L-glutamate
-
 ?
6-acetamido-3-aminohexanoate + 2-oxoglutarate inducible enzyme of lysine catabolism Pseudomonas sp. 6-acetamido-3-oxohexanoate + L-glutamate
-
 ?
6-acetamido-3-aminohexanoate + pyruvate at 6% the rate of 2-oxoglutarate Pseudomonas sp. 6-acetamido-3-oxohexanoate + L-alanine
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
-
 Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
8 9.4 about half-maximal activity at pH 8 and pH 9.4 Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate requirement, pyridoxal phosphate protein Pseudomonas sp.