Cloned (Comment) | Organism |
---|---|
gene ectB, genetic structure, recombinant overexpression of codon-optimized version of (Pl)ectB as N-terminally Strep II-tagged protein from modified plasmid pLC52 in Escherichia coli strain BL21 and TOP 10 cells | Paenibacillus lautus |
Protein Variants | Comment | Organism |
---|---|---|
K274A | site-directed mutagenesis, the mutant is unable to bind cofactor PLP and ins catalytically inactive | Paenibacillus lautus |
K274H | site-directed mutagenesis, the mutant is unable to bind cofactor PLP and ins catalytically inactive. This amino acid substitution does not affect the quaternary assembly of the mutant protein | Paenibacillus lautus |
K274R | site-directed mutagenesis, the mutant is unable to bind cofactor PLP and ins catalytically inactive | Paenibacillus lautus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | activates slightly at up to 350 mM, inhibits by 50% at 1.5 M | Paenibacillus lautus | |
NaCl | activates slightly at up to 350 mM, inhibits by 50% at 1.25 M | Paenibacillus lautus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Paenibacillus lautus | |
0.43 | - |
L-2,4-diaminobutanoate | pH 7.0, 45°C, recombinant enzyme | Paenibacillus lautus | |
9.01 | - |
2-oxoglutarate | pH 7.0, 45°C, recombinant enzyme | Paenibacillus lautus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | activates slightly at up to 350 mM, inhibits by 50% at 1.5 M | Paenibacillus lautus | |
NaCl | activates slightly at up to 350 mM, inhibits by 50% at 1.25 M | Paenibacillus lautus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
200000 | - |
about, recombinant Strep II-tagged enzyme, gel filtration | Paenibacillus lautus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-2,4-diaminobutanoate + 2-oxoglutarate | Paenibacillus lautus | - |
L-aspartate 4-semialdehyde + L-glutamate | - |
? | |
L-2,4-diaminobutanoate + 2-oxoglutarate | Paenibacillus lautus Y412MC10 | - |
L-aspartate 4-semialdehyde + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus lautus | D3EKC0 | isolated from the effluent of a hot spring in the Yellowstone National Park (United States) | - |
Paenibacillus lautus Y412MC10 | D3EKC0 | isolated from the effluent of a hot spring in the Yellowstone National Park (United States) | - |
Purification (Comment) | Organism |
---|---|
recombinant Strep II-tagged enzyme from Escherichia coli by streptavidin-affinity chromatography | Paenibacillus lautus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | growth at temperatures of up to 50°C | Paenibacillus lautus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-2,4-diaminobutanoate + 2-oxoglutarate | - |
Paenibacillus lautus | L-aspartate 4-semialdehyde + L-glutamate | - |
? | |
L-2,4-diaminobutanoate + 2-oxoglutarate | preferred enzyme | Paenibacillus lautus | L-aspartate 4-semialdehyde + L-glutamate | - |
? | |
L-2,4-diaminobutanoate + 2-oxoglutarate | - |
Paenibacillus lautus Y412MC10 | L-aspartate 4-semialdehyde + L-glutamate | - |
? | |
L-2,4-diaminobutanoate + 2-oxoglutarate | preferred enzyme | Paenibacillus lautus Y412MC10 | L-aspartate 4-semialdehyde + L-glutamate | - |
? | |
additional information | (Pl)EctB also catalyzes a GABA-TA-type reaction, EC 2.6.1.19, but with less activity | Paenibacillus lautus | ? | - |
- |
|
additional information | (Pl)EctB also catalyzes a GABA-TA-type reaction, EC 2.6.1.19, but with less activity | Paenibacillus lautus Y412MC10 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 49100, recombinant Strep II-tagged enzyme, SDS-PAGE | Paenibacillus lautus |
Synonyms | Comment | Organism |
---|---|---|
(Pl)EctB | - |
Paenibacillus lautus |
EctB | - |
Paenibacillus lautus |
More | see also EC 2.6.1.19 | Paenibacillus lautus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Paenibacillus lautus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 60 | activity range, profile overview | Paenibacillus lautus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Paenibacillus lautus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10 | 40% of maximal activity within this range | Paenibacillus lautus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | PLP, residue Lys274 attaches the PLP cofactor to enzyme (Pl)EctB. The architecture of the PLP-binding site, formed by two monomeric subunits, overview | Paenibacillus lautus |
General Information | Comment | Organism |
---|---|---|
evolution | EctB-type proteins are highly conserved | Paenibacillus lautus |
additional information | structural homology modeling of (Pl)EctB using the crystal structure of the GABA transaminase from Arthrobacter aurescens (PDB ID 4ATP, EC 2.6.1.19) as template | Paenibacillus lautus |