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Literature summary for 2.6.1.83 extracted from
- Methanococci use the diaminopimelate aminotransferase (DapL) pathway for lysine biosynthesis (2010), J. Bacteriol., 192, 3304-3310.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Origami 2(DE3), also used in complementation experiments | Methanococcus maripaludis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0828 | - |
LL-diaminopimelate | pH 8.5, 70°C | Methanococcus maripaludis |  |
| 0.42 | - |
2-oxoglutarate | pH 8.5, 70°C | Methanococcus maripaludis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47900 | - |
for the protomer | Methanococcus maripaludis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + tetrahydrodipicolinate + H2O | Methanococcus maripaludis | - |
LL-diaminopimelate + 2-oxoglutarate | - |
r | |
| LL-diaminopimelate + 2-oxoglutarate | Methanococcus maripaludis | - |
L-glutamate + tetrahydrodipicolinate + H2O | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanococcus maripaludis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| cell extract for 10 min at 70°C, centrifugation, anion-exchange chromatography | Methanococcus maripaludis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.39 | - |
pH 8.5, 70°C | Methanococcus maripaludis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + tetrahydrodipicolinate + H2O | - |
Methanococcus maripaludis | LL-diaminopimelate + 2-oxoglutarate | - |
r | |
| LL-diaminopimelate + 2-oxoglutarate | - |
Methanococcus maripaludis | L-glutamate + tetrahydrodipicolinate + H2O | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| diaminopimelate aminotransferase | - |
Methanococcus maripaludis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Methanococcus maripaludis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 100 | - |
Methanococcus maripaludis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
pH 8.5, at least for 30 min | Methanococcus maripaludis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.31 | - |
LL-diaminopimelate | after correction for a purity of 70%, pH 8.5, 70°C | Methanococcus maripaludis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Methanococcus maripaludis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | about 40% activity at pH 6.0, about 50% activity at pH 10.0 | Methanococcus maripaludis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Methanococcus maripaludis | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | lysine biosynthesis pathway | Methanococcus maripaludis |
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