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Literature summary for 2.6.1.99 extracted from

  • Tao, Y.; Ferrer, J.L.; Ljung, K.; Pojer, F.; Hong, F.; Long, J.A.; Li, L.; Moreno, J.E.; Bowman, M.E.; Ivans, L.J.; Cheng, Y.; Lim, J.; Zhao, Y.; Ballare, C.L.; Sandberg, G.; Noel, J.P.; Chory, J.
    Rapid synthesis of auxin via a new tryptophan-dependent pathway is required for shade avoidance in plants (2008), Cell, 133, 164-176.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene sav3, DNA and amino acid sequence deterination and analysis, SAV3 has a localized and dynamic expression pattern Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tryptophan + pyruvate Arabidopsis thaliana SAV3 is specific for L-Trp indole-3-pyruvate + L-alanine
-
?
L-tryptophan + pyruvate Arabidopsis thaliana Col-0 SAV3 is specific for L-Trp indole-3-pyruvate + L-alanine
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9S7N2
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-
Arabidopsis thaliana Col-0 Q9S7N2
-
-

Source Tissue

Source Tissue Comment Organism Textmining
hypocotyl
-
Arabidopsis thaliana
-
leaf high expression level of SAV3 Arabidopsis thaliana
-
additional information SAV3 has a localized and dynamic expression pattern Arabidopsis thaliana
-
seedling
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tryptophan + pyruvate SAV3 is specific for L-Trp Arabidopsis thaliana indole-3-pyruvate + L-alanine
-
?
L-tryptophan + pyruvate L-Trp is the preferred substrate Arabidopsis thaliana indole-3-pyruvate + L-alanine
-
?
L-tryptophan + pyruvate SAV3 is specific for L-Trp Arabidopsis thaliana Col-0 indole-3-pyruvate + L-alanine
-
?
L-tryptophan + pyruvate L-Trp is the preferred substrate Arabidopsis thaliana Col-0 indole-3-pyruvate + L-alanine
-
?
additional information SAV3 also uses L-Phe, Tyr, Leu, Ala, Met and Gln as substrates in vitro Arabidopsis thaliana ?
-
?
additional information SAV3 also uses L-Phe, Tyr, Leu, Ala, Met and Gln as substrates in vitro Arabidopsis thaliana Col-0 ?
-
?

Subunits

Subunits Comment Organism
More SAV3 contains a C-terminal alliinase/aminotransferase domain Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
SAV3
-
Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
-
Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction SAV3 mutants are unable to elongate in simulated shade light, phenotypes, overview. Other indole acetic acid biosynthetic pathways cannot compensate for the loss of the SAV3-dependent pathway. sav3 mutants have reduced auxin levels and a diminished auxin response, and sav3 mutants exhibit shorter hypocotyls than WT when grown in simulated shade and partially suppress the constitutive shade avoidance phenotype of a phyB null mutant, sav3-1 plants are shorter and have reduced leaf hyponasty as compared to wild-type plants. The sav3-1 mutant fails to induce shade avoidance syndrome, SAS, in a controlled environment typically used to detect PHYB-mediated SAS responses in light-grown plants Arabidopsis thaliana
additional information SAV3 in silico docking using the crystal structure obtained from SAV3 crystals soaked in L-Phe and co-crystallized with pyridoxal 5'-phosphate, for analyzing substrate and cofactor pecificities, overview Arabidopsis thaliana
physiological function SAV3 is an L-Trp aminotransferase involved in indole 3-acetic acid biosynthesis. SAV3 catalyzes the formation of indole-3-pyruvic acid from L-tryptophan as first step in an auxin biosynthetic pathway. Feedback regulation on SAV3 expression by shade Arabidopsis thaliana