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Literature summary for 2.7.1.100 extracted from

  • Ku, S.Y.; Cornell, K.A.; Howell, P.L.
    Structure of Arabidopsis thaliana 5-methylthioribose kinase reveals a more occluded active site than its bacterial homolog (2007), BMC Struct. Biol., 7, 70.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment) Organism
structure determination, 1.9 A resolution, diffraction data and crystallographic refinement statistics, similarity to enzyme structure of Bacillus subtilis, conformation of Gly-rich loop more extended in Arabidopsis thaliana than in bacterial enzyme homologues, different conformations of G-loops and W-loops in Arabidopsis thaliana and Bacillus subtilis enzymes despite of high sequence similarities, significant ATPase activity in the absence of 5-methyl-5-thio-D-ribose suggested, features of the substrate binding site offer opportunities for a simple organic salt of a 5-methyl-5-thio-D-ribose analog to specifically inhibit kinase activity Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + S-methyl-5-thio-D-ribose Arabidopsis thaliana
-
ADP + 5-methyl-5-thio-D-ribose 1-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9C6D2
-
-

Purification (Commentary)

Purification (Comment) Organism
purified from inclusion bodies, gel filtration Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
mapping of secondary structural elements by in silico analysis, effect of pH on kinase activity, structure compared to enzyme structure of Bacilus subtilis, same protein kinase fold observed in other evolutionary related protein kinase-like phosphotransferases Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + S-methyl-5-thio-D-ribose
-
Arabidopsis thaliana ADP + 5-methyl-5-thio-D-ribose 1-phosphate
-
?
ATP + S-methyl-5-thio-D-ribose structure analysis in Arabidopsis thaliana, provides template for structure-based design of agrochemicals, particularly herbicides with the possibility that effectiveness can be regulated by nutrient levels Arabidopsis thaliana ADP + 5-methyl-5-thio-D-ribose 1-phosphate
-
?

Synonyms

Synonyms Comment Organism
MTR kinase
-
Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
ATP
-
Arabidopsis thaliana