Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Arabidopsis thaliana |
Crystallization (Comment) | Organism |
---|---|
structure determination, 1.9 A resolution, diffraction data and crystallographic refinement statistics, similarity to enzyme structure of Bacillus subtilis, conformation of Gly-rich loop more extended in Arabidopsis thaliana than in bacterial enzyme homologues, different conformations of G-loops and W-loops in Arabidopsis thaliana and Bacillus subtilis enzymes despite of high sequence similarities, significant ATPase activity in the absence of 5-methyl-5-thio-D-ribose suggested, features of the substrate binding site offer opportunities for a simple organic salt of a 5-methyl-5-thio-D-ribose analog to specifically inhibit kinase activity | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + S-methyl-5-thio-D-ribose | Arabidopsis thaliana | - |
ADP + 5-methyl-5-thio-D-ribose 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9C6D2 | - |
- |
Purification (Comment) | Organism |
---|---|
purified from inclusion bodies, gel filtration | Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
mapping of secondary structural elements by in silico analysis, effect of pH on kinase activity, structure compared to enzyme structure of Bacilus subtilis, same protein kinase fold observed in other evolutionary related protein kinase-like phosphotransferases | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + S-methyl-5-thio-D-ribose | - |
Arabidopsis thaliana | ADP + 5-methyl-5-thio-D-ribose 1-phosphate | - |
? | |
ATP + S-methyl-5-thio-D-ribose | structure analysis in Arabidopsis thaliana, provides template for structure-based design of agrochemicals, particularly herbicides with the possibility that effectiveness can be regulated by nutrient levels | Arabidopsis thaliana | ADP + 5-methyl-5-thio-D-ribose 1-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MTR kinase | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Arabidopsis thaliana |