Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus subtilis |
gene yaaG of the yaaG/yaaF operon, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli | Bacillus subtilis |
General Stability | Organism |
---|---|
bovine serum albumin stabilizes the enzyme in solution | Bacillus subtilis |
highly unstable at 0°C in diluted form, no loss of activity in the presence of bovine serum albumin | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dGMP | noncompetitive versus UTP and 2'-deoxyguanosine | Bacillus subtilis | |
additional information | no substrate inhibition by CTP, ATP, and GTP | Bacillus subtilis | |
UDP | competitive versus UTP, noncompetitive versus 2'-deoxyguanosine | Bacillus subtilis | |
UTP | substrate inhibition, overview | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
2'-deoxyguanosine | pH 8.5, 37°C, cosubstrate UTP | Bacillus subtilis | |
0.0006 | - |
2'-deoxyguanosine | pH 8.5, 37°C, recombinant enzyme, with UTP as donor substrate | Bacillus subtilis | |
0.0017 | - |
2'-deoxyguanosine | pH 8.5, 37°C, recombinant enzyme, with CTP as donor substrate | Bacillus subtilis | |
0.006 | - |
UTP | pH 8.5, 37°C | Bacillus subtilis | |
0.006 | - |
UTP | pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate | Bacillus subtilis | |
0.0065 | - |
2'-deoxyguanosine | pH 8.5, 37°C, cosubstrate ATP | Bacillus subtilis | |
0.0065 | - |
2'-deoxyguanosine | pH 8.5, 37°C, recombinant enzyme, with ATP as donor substrate | Bacillus subtilis | |
0.01 | - |
2'-deoxyguanosine | pH 8.5, 37°C, cosubstrate GTP | Bacillus subtilis | |
0.0104 | - |
2'-deoxyguanosine | pH 8.5, 37°C, recombinant enzyme, with GTP as donor substrate | Bacillus subtilis | |
0.017 | - |
2'-deoxyguanosine | pH 8.5, 37°C, cosubstrate CTP | Bacillus subtilis | |
0.035 | - |
CTP | pH 8.5, 37°C | Bacillus subtilis | |
0.035 | - |
CTP | pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate | Bacillus subtilis | |
0.036 | - |
ATP | pH 8.5, 37°C | Bacillus subtilis | |
0.036 | - |
ATP | pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate | Bacillus subtilis | |
0.046 | - |
GTP | pH 8.5, 37°C | Bacillus subtilis | |
0.046 | - |
GTP | pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Bacillus subtilis | |
Mg2+ | MgNTP2- is most probably the true phosphate donor | Bacillus subtilis | |
Mg2+ | required for activity, no inhibition by excess Mg2+, at 5 mM | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24147 | - |
2 * 24147, electrospray mass spectrometry | Bacillus subtilis |
24147 | - |
2 * 24147, recombinant enzyme, electrospray mass spectrometry | Bacillus subtilis |
49000 | - |
recombinant enzyme, gel filtration | Bacillus subtilis |
49000 | - |
recombinant deoxyguanosine kinase, gel filtration | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bacillus subtilis | - |
gene yaaG | - |
Purification (Comment) | Organism |
---|---|
recombinant deoxyguanosine kinase, BlueA dye affinity, phenyl-Sepharose | Bacillus subtilis |
recombinant enzyme from Escherichia coli by affinity and phenyl resin chromatography to homogeneity | Bacillus subtilis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + deoxyguanosine = ADP + dGMP | sequential Bi Bi mechanism | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.54 | - |
enzyme in crude recombinant Escherichia coli cell extract, substrate 2'-deoxyguanosine | Bacillus subtilis |
2.3 | - |
dAdo/dCyd kinase in crude recombinant Escherichia coli cell extract, substrate 2'-deoxyadenosine or 2'-deoxycytidine | Bacillus subtilis |
8.1 | - |
recombinant deoxyguanosine kinase | Bacillus subtilis |
8.1 | - |
purified recombinant enzyme, substrate 2'-deoxyguanosine | Bacillus subtilis |
Storage Stability | Organism |
---|---|
-20°C, more than 1 mg/ml protein, 50% glycerol, 1 year, no loss of activity | Bacillus subtilis |
-20°C, purified recombinant enzyme at 1 mg/ml, Tris-HCl, pH 7.8, 50% v/v glycerol, at least 1 year without loss of activity | Bacillus subtilis |
0°C, the diluted enzyme is highly unstable, but in presence of 1 mg/ml bovine serum albumin, the diluted enzyme is stable for months | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2'-deoxyguanosine | - |
Bacillus subtilis | ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
ATP + deoxyadenosine | 3% of activity with deoxyguanosine | Bacillus subtilis | ADP + dAMP | - |
? | |
ATP + deoxycytidine | recombinant deoxyguanosine kinase, 3% of activity with deoxyguanosine | Bacillus subtilis | ADP + dCMP | - |
? | |
ATP + deoxyinosine | 63% of activity with deoxyguanosine | Bacillus subtilis | ADP + dIMP | - |
? | |
ATP + guanosine | recombinant deoxyguanosine kinase, 7% of activity with deoxyguanosine | Bacillus subtilis | ADP + GMP | - |
? | |
CTP + 2'-deoxyguanosine | - |
Bacillus subtilis | CDP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
GTP + 2'-deoxyadenosine | very low activity | Bacillus subtilis | GDP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
GTP + 2'-deoxycytidine | very low activity | Bacillus subtilis | GDP + 2'-deoxycytidine 5'-phosphate | - |
? | |
GTP + 2'-deoxyguanosine | - |
Bacillus subtilis | GDP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
GTP + 2'-deoxyinosine | - |
Bacillus subtilis | GDP + 2'-deoxyinosine 5'-phosphate | - |
? | |
GTP + guanosine | very low activity | Bacillus subtilis | GDP + guanosine 5'-phosphate | - |
? | |
additional information | substrate specificity, the enzyme utilizes all naturally occurring ribo-and deoxyribonucleosides triphosphates, with the exception of dGTP, as phosphate donors, and shows poor activity with thymidine, inosine, adenosine, 2,3-dideoxyguanosine, 2,3-dideoxyinosine, 2,3-dideoxyadenosine, and hypoxanthine arabinoside as acceptor substrates | Bacillus subtilis | ? | - |
? | |
UTP + 2'-deoxyguanosine | preferred substrates | Bacillus subtilis | UDP + 2'-deoxyguanosine 5'-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 24147, electrospray mass spectrometry | Bacillus subtilis |
dimer | 2 * 24147, recombinant enzyme, electrospray mass spectrometry | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
deoxyguanosine kinase | - |
Bacillus subtilis |
DGK | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.41 | - |
deoxyguanosine | pH 8.5, 37°C, cosubstrate UTP | Bacillus subtilis | |
1.41 | - |
UTP | pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate | Bacillus subtilis | |
1.41 | - |
2'deoxyguanosine | pH 8.5, 37°C, recombinant enzyme, with UTP as donor substrate | Bacillus subtilis | |
1.53 | - |
deoxyguanosine | pH 8.5, 37°C, cosubstrate ATP | Bacillus subtilis | |
1.53 | - |
ATP | pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate | Bacillus subtilis | |
1.53 | - |
2'deoxyguanosine | pH 8.5, 37°C, recombinant enzyme, with ATP as donor substrate | Bacillus subtilis | |
1.69 | - |
deoxyguanosine | pH 8.5, 37°C, cosubstrate GTP | Bacillus subtilis | |
1.69 | - |
GTP | pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate | Bacillus subtilis | |
1.69 | - |
2'deoxyguanosine | pH 8.5, 37°C, recombinant enzyme, with GTP as donor substrate | Bacillus subtilis | |
2.25 | - |
deoxyguanosine | pH 8.5, 37°C, cosubstrate CTP | Bacillus subtilis | |
2.25 | - |
CTP | pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate | Bacillus subtilis | |
2.25 | - |
2'deoxyguanosine | pH 8.5, 37°C, recombinant enzyme, with CTP as donor substrate | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Bacillus subtilis |
9 | - |
broad optimum | Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.6 | 9 | more than 80% of maximal activity at pH 7.5 and pH 11.5, respectively, 60% of maximal activity at pH 6.0, complete loss of activity below pH 5.6 | Bacillus subtilis |
6 | 11.5 | over 80% of maximal activity at pH 7.5 and pH 11.5, about 60% of maximal activity at pH 6.0, enzyme is completely inactive below pH 5.6 | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Bacillus subtilis | |
CTP | - |
Bacillus subtilis | |
GTP | - |
Bacillus subtilis | |
additional information | the enzyme utilizes all naturally occurring ribo-and deoxyribonucleosides triphosphates, with the exception of dGTP, as phosphate donors | Bacillus subtilis | |
UTP | - |
Bacillus subtilis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | product inhibition of the enzyme, overview | Bacillus subtilis | |
0.063 | - |
UDP | pH 8.5, 37°C, cosubstrate UTP | Bacillus subtilis |