Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GTP | stimulates kinase activity | Salmonella enterica subsp. enterica serovar Typhimurium |
Cloned (Comment) | Organism |
---|---|
overexpressed in strain JE3207 | Salmonella enterica subsp. enterica serovar Typhimurium |
General Stability | Organism |
---|---|
substrates provides little protection from inhibition by DTNB | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DTNB | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.066 | - |
5'-deoxyadenosylcobinamide | pH 8.8, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.206 | - |
ATP | pH 8.8, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19700 | - |
predicted mass, based on DNA sequence | Salmonella enterica subsp. enterica serovar Typhimurium |
22000 | - |
2 * 22000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
49000 | - |
gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + adenosylcobinamide | Salmonella enterica subsp. enterica serovar Typhimurium | - |
adenosylcobinamide phosphate + ADP | - |
? | |
ATP + adenosylcobinamide | Salmonella enterica subsp. enterica serovar Typhimurium | assembly of the nucleotide loop of cobalamin | adenosylcobinamide phosphate + ADP | - |
? | |
GTP + adenosylcobinamide | Salmonella enterica subsp. enterica serovar Typhimurium | - |
adenosylcobinamide phosphate + GDP | - |
? | |
GTP + adenosylcobinamide | Salmonella enterica subsp. enterica serovar Typhimurium | assembly of the nucleotide loop of cobalamin | adenosylcobinamide phosphate + GDP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Oxidation Stability | Organism |
---|---|
in presence of oxygen kinase activity is lost | Salmonella enterica subsp. enterica serovar Typhimurium |
Purification (Comment) | Organism |
---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00013 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Storage Stability | Organism |
---|---|
4°C, stored under a N2 headspace, retains 98% of its activity for at least 3 weeks | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-deoxyadenosylcobinamide | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 5'-deoxyadenosylcobinamide phosphate + ADP | - |
? | |
ATP + adenosylcobinamide | - |
Salmonella enterica subsp. enterica serovar Typhimurium | adenosylcobinamide phosphate + ADP | - |
? | |
ATP + adenosylcobinamide | assembly of the nucleotide loop of cobalamin | Salmonella enterica subsp. enterica serovar Typhimurium | adenosylcobinamide phosphate + ADP | - |
? | |
GTP + adenosylcobinamide | - |
Salmonella enterica subsp. enterica serovar Typhimurium | adenosylcobinamide phosphate + GDP | - |
? | |
GTP + adenosylcobinamide | assembly of the nucleotide loop of cobalamin | Salmonella enterica subsp. enterica serovar Typhimurium | adenosylcobinamide phosphate + GDP | - |
? | |
additional information | CobU does not have any detectable ATP:adenosyltransferase activity | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 22000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
Synonyms | Comment | Organism |
---|---|---|
bifunctional CobU enzyme | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
CobU | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
CobU protein | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 60 | retains 50% activity in this range, temperatures above 60°C results in drastic decrease of activity | Salmonella enterica subsp. enterica serovar Typhimurium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.191 | - |
ATP | pH 8.8, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.251 | - |
5'-deoxyadenosylcobinamide | pH 8.8, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | 9 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9.5 | 50% activity retains in this range | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | predicted pI for the denatured protein | - |
5.3 |
Salmonella enterica subsp. enterica serovar Typhimurium | experimetally determined isoelectric point | - |
7.2 |
Salmonella enterica subsp. enterica serovar Typhimurium | experimetally determined isoelectric point | - |
7.5 |