Cloned (Comment) | Organism |
---|---|
gene TM 1469, expression in Escherichia coli strain BL21(DE3) | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Thermotoga maritima | |
0.038 | - |
D-glucose | pH 8.0, 85°C, recombinant enzyme | Thermotoga maritima | |
0.189 | - |
ATP | pH 8.0, 85°C, recombinant enzyme | Thermotoga maritima |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | at 4.4 mM MgCl2 | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
recombinant enzyme, native PAGE | Thermotoga maritima |
33500 | - |
2 * 33500, the recombinant enzyme probably is a dimer, SDS-PAGE | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | Thermotoga maritima | - |
ADP + D-glucose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
gene TM 1469 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) by heat treatment and Ni2+ chelating affinity chromatography | Thermotoga maritima |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
166 | - |
purified recombinant enzyme | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | - |
Thermotoga maritima | ADP + D-glucose 6-phosphate | - |
? | |
additional information | no activity with fructose, galactose, and mannose, development of a coupled assay method with glucose 6-phosphate dehydrogenase stable at pH 6.8-8.5 and 75-90°C, overview | Thermotoga maritima | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 33500, the recombinant enzyme probably is a dimer, SDS-PAGE | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
glk | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Thermotoga maritima |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
development of an assay method stable at pH 6.8-8.5 and 75-90°C, overview | Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
92.6 | - |
D-glucose | pH 8.0, 85°C, recombinant enzyme | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
sharp optimum | Thermotoga maritima |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
development of an assay method stable at pH 6.8-8.5 and 75-90°C, overview | Thermotoga maritima |
7 | 8.5 | - |
Thermotoga maritima |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Thermotoga maritima |